Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29187 | 3' | -50 | NC_006150.1 | + | 44227 | 0.71 | 0.966445 |
Target: 5'- -aCAACCAuUGUAGCucACCGCGAuGGGGa -3' miRNA: 3'- aaGUUGGU-GUAUUGu-UGGCGCU-CCUC- -5' |
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29187 | 3' | -50 | NC_006150.1 | + | 2233 | 0.72 | 0.937859 |
Target: 5'- gUUCAGCUGCAggcgagGGCcguGGCCGCcGAGGAGg -3' miRNA: 3'- -AAGUUGGUGUa-----UUG---UUGGCG-CUCCUC- -5' |
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29187 | 3' | -50 | NC_006150.1 | + | 182258 | 0.73 | 0.903674 |
Target: 5'- -aCAACCGCGUGACAcggcgaccgGCgGCGAGGc- -3' miRNA: 3'- aaGUUGGUGUAUUGU---------UGgCGCUCCuc -5' |
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29187 | 3' | -50 | NC_006150.1 | + | 126117 | 0.74 | 0.897101 |
Target: 5'- cUCAAgUACGaa--GACCGCGAGGAGg -3' miRNA: 3'- aAGUUgGUGUauugUUGGCGCUCCUC- -5' |
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29187 | 3' | -50 | NC_006150.1 | + | 106257 | 0.75 | 0.826686 |
Target: 5'- cUCAGCUgacggcaACAgccGCGGCCGCGGGGGGa -3' miRNA: 3'- aAGUUGG-------UGUau-UGUUGGCGCUCCUC- -5' |
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29187 | 3' | -50 | NC_006150.1 | + | 103712 | 0.78 | 0.681532 |
Target: 5'- cUCGcggGCAUGACGGCCGCGGGGAGu -3' miRNA: 3'- aAGUuggUGUAUUGUUGGCGCUCCUC- -5' |
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29187 | 3' | -50 | NC_006150.1 | + | 103994 | 1.08 | 0.017386 |
Target: 5'- gUUCAACCACAUAACAACCGCGAGGAGa -3' miRNA: 3'- -AAGUUGGUGUAUUGUUGGCGCUCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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