Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29188 | 3' | -55.8 | NC_006150.1 | + | 90908 | 0.7 | 0.799866 |
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Target: 5'- aGCCUGG-----GGUGCGGCagucacaACAGCCu -3' miRNA: 3'- -UGGACCuuaacCCACGUCGg------UGUCGG- -5' |
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| 29188 | 3' | -55.8 | NC_006150.1 | + | 193599 | 0.7 | 0.790943 |
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Target: 5'- gACgCUGGcgAGUgaaGGUGUuGCCGCAGCCa -3' miRNA: 3'- -UG-GACC--UUAac-CCACGuCGGUGUCGG- -5' |
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| 29188 | 3' | -55.8 | NC_006150.1 | + | 1626 | 0.73 | 0.625022 |
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Target: 5'- uGCUgcgcaGGGcgUGGGcguagGCAGUCACGGCCu -3' miRNA: 3'- -UGGa----CCUuaACCCa----CGUCGGUGUCGG- -5' |
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| 29188 | 3' | -55.8 | NC_006150.1 | + | 156620 | 0.73 | 0.624011 |
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Target: 5'- gGCCUGGGAUcUGaaagccGUGCAGCCACAcgauauuGCCc -3' miRNA: 3'- -UGGACCUUA-ACc-----CACGUCGGUGU-------CGG- -5' |
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| 29188 | 3' | -55.8 | NC_006150.1 | + | 147350 | 0.75 | 0.525338 |
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Target: 5'- cCCUGGAg--GGGcacgGCaaaAGCCGCGGCCg -3' miRNA: 3'- uGGACCUuaaCCCa---CG---UCGGUGUCGG- -5' |
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| 29188 | 3' | -55.8 | NC_006150.1 | + | 104359 | 1.12 | 0.002448 |
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Target: 5'- cACCUGGAAUUGGGUGCAGCCACAGCCg -3' miRNA: 3'- -UGGACCUUAACCCACGUCGGUGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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