Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29189 | 3' | -54.3 | NC_006150.1 | + | 102879 | 0.68 | 0.933619 |
Target: 5'- gGCGCCUACuGGCGggcaagGUGGgucGugAUGUAc -3' miRNA: 3'- -UGUGGGUG-CCGUa-----CACCa--CugUACAU- -5' |
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29189 | 3' | -54.3 | NC_006150.1 | + | 109812 | 0.68 | 0.9175 |
Target: 5'- gAUGuuC-CGGCGUGUGGUGugGUGg- -3' miRNA: 3'- -UGUggGuGCCGUACACCACugUACau -5' |
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29189 | 3' | -54.3 | NC_006150.1 | + | 91069 | 0.75 | 0.589518 |
Target: 5'- gGCACCCGCGGUgauGUGUGcugGACAUGa- -3' miRNA: 3'- -UGUGGGUGCCG---UACACca-CUGUACau -5' |
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29189 | 3' | -54.3 | NC_006150.1 | + | 105163 | 1.07 | 0.007377 |
Target: 5'- cACACCCACGGCAUGUGGUGACAUGUAa -3' miRNA: 3'- -UGUGGGUGCCGUACACCACUGUACAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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