miRNA: 3'- -GGACGCG-GAGCaGACGUGcGUUGUGU- -5'
miRNA: 3'- ggaCGCGGAG-CAG----ACGUGCGUUGUGu -5'
miRNA: 3'- -GGACGCGGAGCAGACGugCGUugUGu -5'
miRNA: 3'- ggaCGCGGAGCAGACG---------------UGCGU-UGUGU- -5'
miRNA: 3'- -GGACGCGGAGCAGACGUGCGUUGUGU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29192 | 3' | -55.7 | NC_006150.1 | + | 217624 | 0.72 | 0.72121 |
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Target: 5'- aCCUGUGCaUUUGcCUGCGC-CAGCGCAg -3' miRNA: 3'- -GGACGCG-GAGCaGACGUGcGUUGUGU- -5' |
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| 29192 | 3' | -55.7 | NC_006150.1 | + | 187770 | 0.72 | 0.72121 |
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Target: 5'- --cGCGCCUCaGUCuuccUGCACGUGACAUc -3' miRNA: 3'- ggaCGCGGAG-CAG----ACGUGCGUUGUGu -5' |
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| 29192 | 3' | -55.7 | NC_006150.1 | + | 218710 | 0.73 | 0.662056 |
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Target: 5'- cCCUGCGCCUUGUUUGU--GCAugAUg -3' miRNA: 3'- -GGACGCGGAGCAGACGugCGUugUGu -5' |
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| 29192 | 3' | -55.7 | NC_006150.1 | + | 13142 | 0.75 | 0.566321 |
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Target: 5'- --gGCGCCUUGUCUGUuauuaaggugccugaGCGCAcACACAa -3' miRNA: 3'- ggaCGCGGAGCAGACG---------------UGCGU-UGUGU- -5' |
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| 29192 | 3' | -55.7 | NC_006150.1 | + | 109440 | 1.1 | 0.003769 |
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Target: 5'- uCCUGCGCCUCGUCUGCACGCAACACAg -3' miRNA: 3'- -GGACGCGGAGCAGACGUGCGUUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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