Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29193 | 5' | -53.1 | NC_006150.1 | + | 217848 | 0.66 | 0.986901 |
Target: 5'- cUUGAgugACAcuuGGCUCgGUGCCGUUGUGc -3' miRNA: 3'- cAGUUa--UGU---UCGGGgUACGGCGACAC- -5' |
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29193 | 5' | -53.1 | NC_006150.1 | + | 91979 | 0.66 | 0.981396 |
Target: 5'- --aGAUGCAAGCUCUcgGCCG-UGUa -3' miRNA: 3'- cagUUAUGUUCGGGGuaCGGCgACAc -5' |
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29193 | 5' | -53.1 | NC_006150.1 | + | 119689 | 0.67 | 0.977829 |
Target: 5'- gGUCGAUGCGccucaguuuugcggaGGCCCUcUGCUGUaUGUGc -3' miRNA: 3'- -CAGUUAUGU---------------UCGGGGuACGGCG-ACAC- -5' |
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29193 | 5' | -53.1 | NC_006150.1 | + | 41462 | 0.67 | 0.971569 |
Target: 5'- uUCAGUGCAucuGCCUgccacacgcaCAUGCCGgUGUa -3' miRNA: 3'- cAGUUAUGUu--CGGG----------GUACGGCgACAc -5' |
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29193 | 5' | -53.1 | NC_006150.1 | + | 414 | 0.69 | 0.926662 |
Target: 5'- uGUCAcUGC-GGCCC--UGCUGCUGUGu -3' miRNA: 3'- -CAGUuAUGuUCGGGguACGGCGACAC- -5' |
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29193 | 5' | -53.1 | NC_006150.1 | + | 64412 | 0.74 | 0.705464 |
Target: 5'- uGUCuuGUACAGGCaaCGUGCCGCUGa- -3' miRNA: 3'- -CAGu-UAUGUUCGggGUACGGCGACac -5' |
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29193 | 5' | -53.1 | NC_006150.1 | + | 110733 | 1.09 | 0.006504 |
Target: 5'- uGUCAAUACAAGCCCCAUGCCGCUGUGg -3' miRNA: 3'- -CAGUUAUGUUCGGGGUACGGCGACAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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