Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29196 | 3' | -66.6 | NC_006150.1 | + | 88736 | 0.66 | 0.576236 |
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Target: 5'- -aGGGcUCGuCGGGGCa--GCCGGCUGc -3' miRNA: 3'- agCCCuGGC-GCCCCGgagCGGUCGGC- -5' |
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| 29196 | 3' | -66.6 | NC_006150.1 | + | 182558 | 0.66 | 0.53184 |
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Target: 5'- cUCGGGgggcACCcgggggguucgcggaGCGGGgacauguuuguuauGCCUCGCC-GCCGg -3' miRNA: 3'- -AGCCC----UGG---------------CGCCC--------------CGGAGCGGuCGGC- -5' |
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| 29196 | 3' | -66.6 | NC_006150.1 | + | 498 | 0.67 | 0.487012 |
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Target: 5'- cCGGGGCCGCGGauaGCCcUCGCCcuUCu -3' miRNA: 3'- aGCCCUGGCGCCc--CGG-AGCGGucGGc -5' |
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| 29196 | 3' | -66.6 | NC_006150.1 | + | 62727 | 0.69 | 0.404077 |
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Target: 5'- aCGGGACCGCGgagacccGGGaCCUCGauGGCUa -3' miRNA: 3'- aGCCCUGGCGC-------CCC-GGAGCggUCGGc -5' |
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| 29196 | 3' | -66.6 | NC_006150.1 | + | 154588 | 0.69 | 0.359943 |
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Target: 5'- aCGGcGGCCGCGaGGGCCagaacgUGCCcgguGCCa -3' miRNA: 3'- aGCC-CUGGCGC-CCCGGa-----GCGGu---CGGc -5' |
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| 29196 | 3' | -66.6 | NC_006150.1 | + | 132690 | 0.69 | 0.359225 |
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Target: 5'- cCGcGACCGCGGagacaaacuccucGGCCacCGCCAGCCc -3' miRNA: 3'- aGCcCUGGCGCC-------------CCGGa-GCGGUCGGc -5' |
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| 29196 | 3' | -66.6 | NC_006150.1 | + | 88605 | 0.71 | 0.297468 |
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Target: 5'- -gGGGACCGCacagcucgcaggugGGGGCgUCGCgaGGCCc -3' miRNA: 3'- agCCCUGGCG--------------CCCCGgAGCGg-UCGGc -5' |
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| 29196 | 3' | -66.6 | NC_006150.1 | + | 113907 | 1.07 | 0.000824 |
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Target: 5'- uUCGGGACCGCGGGGCCUCGCCAGCCGc -3' miRNA: 3'- -AGCCCUGGCGCCCCGGAGCGGUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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