Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29199 | 3' | -53.2 | NC_006150.1 | + | 147590 | 0.66 | 0.987027 |
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Target: 5'- -aGCgagagCGACUC-GCgGCAGCUCu- -3' miRNA: 3'- gaCGaaa--GCUGAGaCGaCGUCGAGcu -5' |
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| 29199 | 3' | -53.2 | NC_006150.1 | + | 130475 | 0.68 | 0.955192 |
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Target: 5'- aUGCgucaggCGGCUaugGCUGCGGCggugCGAg -3' miRNA: 3'- gACGaaa---GCUGAga-CGACGUCGa---GCU- -5' |
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| 29199 | 3' | -53.2 | NC_006150.1 | + | 55180 | 0.71 | 0.854794 |
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Target: 5'- aUGCaugaCGACg--GCUGCGGCUCGGg -3' miRNA: 3'- gACGaaa-GCUGagaCGACGUCGAGCU- -5' |
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| 29199 | 3' | -53.2 | NC_006150.1 | + | 114057 | 0.74 | 0.738429 |
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Target: 5'- -gGCUgagCGGCUCgaGCUGCAGCagagUCGAa -3' miRNA: 3'- gaCGAaa-GCUGAGa-CGACGUCG----AGCU- -5' |
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| 29199 | 3' | -53.2 | NC_006150.1 | + | 185104 | 0.76 | 0.60624 |
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Target: 5'- gUGCUUggCGGCguugcGCUGCAGCUCGGa -3' miRNA: 3'- gACGAAa-GCUGaga--CGACGUCGAGCU- -5' |
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| 29199 | 3' | -53.2 | NC_006150.1 | + | 114315 | 1.1 | 0.006198 |
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Target: 5'- cCUGCUUUCGACUCUGCUGCAGCUCGAg -3' miRNA: 3'- -GACGAAAGCUGAGACGACGUCGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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