miRNA: 3'- -UG-UCUUgUCGACUAUCUca--CGGGGAC- -5'
miRNA: 3'- -UGUcuUGu--CGACuaUCUCACGGGGAc -5'
miRNA: 3'- -UGUCU---UGUCGACUauCUCaCGGGGac -5'
miRNA: 3'- ugUCUUG-UCGACUaUCUCaCGGGGac -5'
miRNA: 3'- -UGUCUUGUCGACUAUCUCACGGGGAC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29201 | 3' | -53.4 | NC_006150.1 | + | 132573 | 0.66 | 0.982353 |
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Target: 5'- aGCgAGAGgAGCUGGUGGAcgagGUCUCUGa -3' miRNA: 3'- -UG-UCUUgUCGACUAUCUca--CGGGGAC- -5' |
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| 29201 | 3' | -53.4 | NC_006150.1 | + | 87603 | 0.67 | 0.967005 |
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Target: 5'- cGCAu-GCuguGCUGugGGAGUGCCCCUc -3' miRNA: 3'- -UGUcuUGu--CGACuaUCUCACGGGGAc -5' |
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| 29201 | 3' | -53.4 | NC_006150.1 | + | 113651 | 0.68 | 0.944011 |
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Target: 5'- gACGGAgauGCGGCUGGc-GAG-GCCCCg- -3' miRNA: 3'- -UGUCU---UGUCGACUauCUCaCGGGGac -5' |
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| 29201 | 3' | -53.4 | NC_006150.1 | + | 160252 | 0.7 | 0.864832 |
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Target: 5'- -gAGAGCgAGCUGGaAGAGgGCCCCa- -3' miRNA: 3'- ugUCUUG-UCGACUaUCUCaCGGGGac -5' |
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| 29201 | 3' | -53.4 | NC_006150.1 | + | 115730 | 1.07 | 0.007905 |
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Target: 5'- cACAGAACAGCUGAUAGAGUGCCCCUGc -3' miRNA: 3'- -UGUCUUGUCGACUAUCUCACGGGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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