Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29204 | 5' | -61.9 | NC_006150.1 | + | 33948 | 0.66 | 0.739508 |
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Target: 5'- aGGuACaGUGCCCCccgguuaguGUAUGG-GGCUAUGg -3' miRNA: 3'- gCC-UG-CACGGGG---------CGUACCgCCGGUAC- -5' |
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| 29204 | 5' | -61.9 | NC_006150.1 | + | 180652 | 0.66 | 0.738592 |
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Target: 5'- uGGuauugcuCGUGUuguuuCCCGCAgcgcacgugcuguUGGCGGCCAa- -3' miRNA: 3'- gCCu------GCACG-----GGGCGU-------------ACCGCCGGUac -5' |
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| 29204 | 5' | -61.9 | NC_006150.1 | + | 181280 | 0.66 | 0.730316 |
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Target: 5'- aGGGCGggagaggcggcUGCCCUGgGcGGCGGCgGUa -3' miRNA: 3'- gCCUGC-----------ACGGGGCgUaCCGCCGgUAc -5' |
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| 29204 | 5' | -61.9 | NC_006150.1 | + | 130461 | 0.66 | 0.721049 |
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Target: 5'- aGcGACGaGCCUggaUGCGUcaGGCGGCUAUGg -3' miRNA: 3'- gC-CUGCaCGGG---GCGUA--CCGCCGGUAC- -5' |
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| 29204 | 5' | -61.9 | NC_006150.1 | + | 317 | 0.67 | 0.711714 |
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Target: 5'- aGGACGgcUGCCCgGgGuUGGCGGUCu-- -3' miRNA: 3'- gCCUGC--ACGGGgCgU-ACCGCCGGuac -5' |
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| 29204 | 5' | -61.9 | NC_006150.1 | + | 191288 | 0.67 | 0.664298 |
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Target: 5'- aGGAgCGcaGCCCgaauaGCGUGGCGGUCAa- -3' miRNA: 3'- gCCU-GCa-CGGGg----CGUACCGCCGGUac -5' |
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| 29204 | 5' | -61.9 | NC_006150.1 | + | 71101 | 0.7 | 0.493558 |
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Target: 5'- aGGACGgcggGaCCuaGCugcuguaGUGGCGGCCGUGg -3' miRNA: 3'- gCCUGCa---C-GGggCG-------UACCGCCGGUAC- -5' |
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| 29204 | 5' | -61.9 | NC_006150.1 | + | 117312 | 1.08 | 0.001511 |
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Target: 5'- uCGGACGUGCCCCGCAUGGCGGCCAUGg -3' miRNA: 3'- -GCCUGCACGGGGCGUACCGCCGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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