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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29205 | 5' | -57 | NC_006150.1 | + | 88099 | 0.66 | 0.931121 |
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Target: 5'- ---gGUCGCCacuggcgauUGCC-CGGAcggcGCCGCGGa -3' miRNA: 3'- cacaCAGUGG---------ACGGaGCCU----UGGCGCU- -5' |
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| 29205 | 5' | -57 | NC_006150.1 | + | 93024 | 0.66 | 0.92604 |
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Target: 5'- cUGUGg-GCCaGCCuUUGGAGCCGCu- -3' miRNA: 3'- cACACagUGGaCGG-AGCCUUGGCGcu -5' |
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| 29205 | 5' | -57 | NC_006150.1 | + | 112998 | 0.66 | 0.924472 |
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Target: 5'- -gGUGUCGCCaaagucagacaacaUgGCCUCGcGACCGCa- -3' miRNA: 3'- caCACAGUGG--------------A-CGGAGCcUUGGCGcu -5' |
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| 29205 | 5' | -57 | NC_006150.1 | + | 33141 | 0.67 | 0.909451 |
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Target: 5'- -----cCGCCUccGCUUCGGAGCCGCu- -3' miRNA: 3'- cacacaGUGGA--CGGAGCCUUGGCGcu -5' |
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| 29205 | 5' | -57 | NC_006150.1 | + | 220313 | 0.67 | 0.897282 |
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Target: 5'- ---aGUCAUCgGCaUCGGuAGCCGCGAg -3' miRNA: 3'- cacaCAGUGGaCGgAGCC-UUGGCGCU- -5' |
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| 29205 | 5' | -57 | NC_006150.1 | + | 29133 | 0.68 | 0.855694 |
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Target: 5'- gGUGguUGUgACCUGaCaugaUCGGAACCGCa- -3' miRNA: 3'- -CAC--ACAgUGGAC-Gg---AGCCUUGGCGcu -5' |
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| 29205 | 5' | -57 | NC_006150.1 | + | 106490 | 0.68 | 0.848068 |
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Target: 5'- aGUGUuUCACCUGCCgagcaGGAGgCGUGc -3' miRNA: 3'- -CACAcAGUGGACGGag---CCUUgGCGCu -5' |
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| 29205 | 5' | -57 | NC_006150.1 | + | 117409 | 1.09 | 0.003514 |
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Target: 5'- cGUGUGUCACCUGCCUCGGAACCGCGAa -3' miRNA: 3'- -CACACAGUGGACGGAGCCUUGGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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