Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29208 | 3' | -57.5 | NC_006150.1 | + | 9185 | 0.67 | 0.895462 |
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Target: 5'- cCUUCUgAGGGcgGGGGguacgUUCCUGCCGc -3' miRNA: 3'- aGAAGGgUUCCaaCCCU-----AGGGGCGGC- -5' |
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| 29208 | 3' | -57.5 | NC_006150.1 | + | 169769 | 0.67 | 0.875556 |
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Target: 5'- aCUgugcgCCUAAG-UUGGGAUUCCaUGCCGa -3' miRNA: 3'- aGAa----GGGUUCcAACCCUAGGG-GCGGC- -5' |
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| 29208 | 3' | -57.5 | NC_006150.1 | + | 48028 | 0.67 | 0.868513 |
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Target: 5'- uUCUUCugCCAAacuGUcucUGGGGUCCUCGCCu -3' miRNA: 3'- -AGAAG--GGUUc--CA---ACCCUAGGGGCGGc -5' |
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| 29208 | 3' | -57.5 | NC_006150.1 | + | 135650 | 0.68 | 0.838424 |
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Target: 5'- --aUCCCAGGagUGGGcuAUCCCUGUCGu -3' miRNA: 3'- agaAGGGUUCcaACCC--UAGGGGCGGC- -5' |
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| 29208 | 3' | -57.5 | NC_006150.1 | + | 157718 | 0.7 | 0.761275 |
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Target: 5'- aUCUUCCCAacuuGGGUucuucucuuacUGGGGUCuCCCaCCu -3' miRNA: 3'- -AGAAGGGU----UCCA-----------ACCCUAG-GGGcGGc -5' |
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| 29208 | 3' | -57.5 | NC_006150.1 | + | 119538 | 1.09 | 0.003108 |
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Target: 5'- uUCUUCCCAAGGUUGGGAUCCCCGCCGa -3' miRNA: 3'- -AGAAGGGUUCCAACCCUAGGGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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