Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2921 | 3' | -54.6 | NC_001493.1 | + | 18551 | 0.66 | 0.944413 |
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Target: 5'- -cCGUggCC-GUGGACGGGGGuuucaaacuGACCAu -3' miRNA: 3'- ccGCAa-GGaCAUCUGCCCCUu--------CUGGU- -5' |
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| 2921 | 3' | -54.6 | NC_001493.1 | + | 134106 | 0.66 | 0.944413 |
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Target: 5'- -cCGUggCC-GUGGACGGGGGuuucaaacuGACCAu -3' miRNA: 3'- ccGCAa-GGaCAUCUGCCCCUu--------CUGGU- -5' |
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| 2921 | 3' | -54.6 | NC_001493.1 | + | 106045 | 0.66 | 0.939776 |
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Target: 5'- cGCGUUCgUGUGGGuCGGuGGucauGAUCAc -3' miRNA: 3'- cCGCAAGgACAUCU-GCC-CCuu--CUGGU- -5' |
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| 2921 | 3' | -54.6 | NC_001493.1 | + | 66001 | 0.66 | 0.924407 |
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Target: 5'- gGGCGUUCgUcggGGACGuGGGGuaggaGGGCCu -3' miRNA: 3'- -CCGCAAGgAca-UCUGC-CCCU-----UCUGGu -5' |
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| 2921 | 3' | -54.6 | NC_001493.1 | + | 18827 | 0.69 | 0.833329 |
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Target: 5'- uGCGUUCCgggUGUGGugcgaGCGGGGGgugAGACUc -3' miRNA: 3'- cCGCAAGG---ACAUC-----UGCCCCU---UCUGGu -5' |
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| 2921 | 3' | -54.6 | NC_001493.1 | + | 32225 | 0.69 | 0.82484 |
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Target: 5'- aGGCucUUCgUGUGGACGGaGGGAuACCAc -3' miRNA: 3'- -CCGc-AAGgACAUCUGCC-CCUUcUGGU- -5' |
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| 2921 | 3' | -54.6 | NC_001493.1 | + | 106720 | 0.7 | 0.789178 |
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Target: 5'- gGGCa-UUCUGUGacGCGGGGggGACCc -3' miRNA: 3'- -CCGcaAGGACAUc-UGCCCCuuCUGGu -5' |
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| 2921 | 3' | -54.6 | NC_001493.1 | + | 79625 | 0.7 | 0.760885 |
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Target: 5'- gGGCGUUCUUc-AGaACGGGG-AGACCu -3' miRNA: 3'- -CCGCAAGGAcaUC-UGCCCCuUCUGGu -5' |
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| 2921 | 3' | -54.6 | NC_001493.1 | + | 113102 | 0.72 | 0.660441 |
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Target: 5'- cGGUGUuuUUCUGUGGugGGGG-GGuCCGa -3' miRNA: 3'- -CCGCA--AGGACAUCugCCCCuUCuGGU- -5' |
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| 2921 | 3' | -54.6 | NC_001493.1 | + | 47657 | 1.11 | 0.00278 |
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Target: 5'- aGGCGUUCCUGUAGACGGGGAAGACCAu -3' miRNA: 3'- -CCGCAAGGACAUCUGCCCCUUCUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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