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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29211 | 3' | -59.4 | NC_006150.1 | + | 96948 | 0.66 | 0.854627 |
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Target: 5'- aCACGUuccaagccaucACGCCuGGCaCCaUCGAGGGUGa -3' miRNA: 3'- aGUGCG-----------UGUGG-CUG-GGgAGCUCCCAU- -5' |
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| 29211 | 3' | -59.4 | NC_006150.1 | + | 12527 | 0.67 | 0.789736 |
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Target: 5'- cCACaGC-CGCCGGCCCCUCa--GGUGa -3' miRNA: 3'- aGUG-CGuGUGGCUGGGGAGcucCCAU- -5' |
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| 29211 | 3' | -59.4 | NC_006150.1 | + | 84426 | 0.67 | 0.789736 |
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Target: 5'- -gACGUcgGCGCUGACCCCUCGuccgcuuuGGUGu -3' miRNA: 3'- agUGCG--UGUGGCUGGGGAGCuc------CCAU- -5' |
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| 29211 | 3' | -59.4 | NC_006150.1 | + | 119565 | 0.68 | 0.74475 |
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Target: 5'- aC-CGCGCugCGGCCCCgcaccugCGAGaGGa- -3' miRNA: 3'- aGuGCGUGugGCUGGGGa------GCUC-CCau -5' |
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| 29211 | 3' | -59.4 | NC_006150.1 | + | 125475 | 0.69 | 0.69752 |
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Target: 5'- -gACGguCGCGCCGACCUUUCGGGuGGUu -3' miRNA: 3'- agUGC--GUGUGGCUGGGGAGCUC-CCAu -5' |
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| 29211 | 3' | -59.4 | NC_006150.1 | + | 114898 | 0.71 | 0.580827 |
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Target: 5'- cUCGCGCACGagCGGgCCCgaaaagagCGGGGGUAg -3' miRNA: 3'- -AGUGCGUGUg-GCUgGGGa-------GCUCCCAU- -5' |
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| 29211 | 3' | -59.4 | NC_006150.1 | + | 86181 | 0.72 | 0.542445 |
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Target: 5'- cCugGCGCGCCGcCCCCUuCGAcGGUu -3' miRNA: 3'- aGugCGUGUGGCuGGGGA-GCUcCCAu -5' |
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| 29211 | 3' | -59.4 | NC_006150.1 | + | 125061 | 1.07 | 0.002957 |
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Target: 5'- uUCACGCACACCGACCCCUCGAGGGUAg -3' miRNA: 3'- -AGUGCGUGUGGCUGGGGAGCUCCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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