Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29212 | 3' | -55.4 | NC_006150.1 | + | 126021 | 0.66 | 0.95975 |
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Target: 5'- gGUGGUGuGCUGCGacucGGCUCcgucUGGGAu -3' miRNA: 3'- gUAUCGCuCGGCGCa---CCGAGu---ACUCU- -5' |
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| 29212 | 3' | -55.4 | NC_006150.1 | + | 220855 | 0.66 | 0.95975 |
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Target: 5'- uGUGGUGuAGCgGCGagGGUUgAUGAGGg -3' miRNA: 3'- gUAUCGC-UCGgCGCa-CCGAgUACUCU- -5' |
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| 29212 | 3' | -55.4 | NC_006150.1 | + | 115711 | 0.66 | 0.958312 |
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Target: 5'- --gAGCGAGCCGCGguuggacgcccaaGGgUUGUGGGu -3' miRNA: 3'- guaUCGCUCGGCGCa------------CCgAGUACUCu -5' |
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| 29212 | 3' | -55.4 | NC_006150.1 | + | 216632 | 0.66 | 0.943774 |
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Target: 5'- cCGUGGCG-GCUGCG-GuGCUCGgcaGGGAc -3' miRNA: 3'- -GUAUCGCuCGGCGCaC-CGAGUa--CUCU- -5' |
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| 29212 | 3' | -55.4 | NC_006150.1 | + | 91897 | 0.68 | 0.91293 |
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Target: 5'- cCGUAGcCGGGCUGCGgcaGCUCAUGu-- -3' miRNA: 3'- -GUAUC-GCUCGGCGCac-CGAGUACucu -5' |
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| 29212 | 3' | -55.4 | NC_006150.1 | + | 106056 | 0.68 | 0.887753 |
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Target: 5'- --cAGCGGGCCGCGUGaCUCGc---- -3' miRNA: 3'- guaUCGCUCGGCGCACcGAGUacucu -5' |
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| 29212 | 3' | -55.4 | NC_006150.1 | + | 64721 | 0.71 | 0.782624 |
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Target: 5'- aCAUGGUgauguugGAGCUGC--GGCUCGUGAGGu -3' miRNA: 3'- -GUAUCG-------CUCGGCGcaCCGAGUACUCU- -5' |
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| 29212 | 3' | -55.4 | NC_006150.1 | + | 48893 | 0.71 | 0.774371 |
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Target: 5'- uCAUGGUGcGuuGCGUGGCUCGUa--- -3' miRNA: 3'- -GUAUCGCuCggCGCACCGAGUAcucu -5' |
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| 29212 | 3' | -55.4 | NC_006150.1 | + | 125211 | 1.08 | 0.004904 |
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Target: 5'- gCAUAGCGAGCCGCGUGGCUCAUGAGAu -3' miRNA: 3'- -GUAUCGCUCGGCGCACCGAGUACUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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