Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29212 | 5' | -54.8 | NC_006150.1 | + | 155333 | 0.71 | 0.771762 |
Target: 5'- aCACCUCAGCCAGaUACucuaGGUUCCuagcgGGUGGg -3' miRNA: 3'- -GUGGAGUCGGUC-GUG----UCGAGG-----UUACU- -5' |
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29212 | 5' | -54.8 | NC_006150.1 | + | 168544 | 0.71 | 0.771762 |
Target: 5'- gACC-CAGCCgGGCACGGCUcgcacaccgucCCGGUGGu -3' miRNA: 3'- gUGGaGUCGG-UCGUGUCGA-----------GGUUACU- -5' |
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29212 | 5' | -54.8 | NC_006150.1 | + | 88517 | 0.71 | 0.762319 |
Target: 5'- cCGCC-CAGCCgAGCGCAcGCUCCGc--- -3' miRNA: 3'- -GUGGaGUCGG-UCGUGU-CGAGGUuacu -5' |
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29212 | 5' | -54.8 | NC_006150.1 | + | 136342 | 0.73 | 0.652837 |
Target: 5'- gGCCUCGauaucuagcGCCGGCGCGGCUuCCAG-GAu -3' miRNA: 3'- gUGGAGU---------CGGUCGUGUCGA-GGUUaCU- -5' |
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29212 | 5' | -54.8 | NC_006150.1 | + | 125177 | 1.09 | 0.004644 |
Target: 5'- aCACCUCAGCCAGCACAGCUCCAAUGAc -3' miRNA: 3'- -GUGGAGUCGGUCGUGUCGAGGUUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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