Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29214 | 5' | -59.1 | NC_006150.1 | + | 218991 | 0.66 | 0.868828 |
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Target: 5'- -gUCGUCGGaaCGGaCGAUUGGCGCGa -3' miRNA: 3'- uaGGCGGCCgaGCCaGCUGACUGUGU- -5' |
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| 29214 | 5' | -59.1 | NC_006150.1 | + | 15079 | 0.66 | 0.868828 |
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Target: 5'- -gCCGUCGGUUCGGUCGuuaGuuGCAu -3' miRNA: 3'- uaGGCGGCCGAGCCAGCugaCugUGU- -5' |
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| 29214 | 5' | -59.1 | NC_006150.1 | + | 89043 | 0.66 | 0.854235 |
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Target: 5'- -gCCGCCGGCUauG-CGACggcGACAUc -3' miRNA: 3'- uaGGCGGCCGAgcCaGCUGa--CUGUGu -5' |
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| 29214 | 5' | -59.1 | NC_006150.1 | + | 86487 | 0.66 | 0.846659 |
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Target: 5'- aGUUCGCCGGCcuggCGGagGG-UGGCGCGg -3' miRNA: 3'- -UAGGCGGCCGa---GCCagCUgACUGUGU- -5' |
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| 29214 | 5' | -59.1 | NC_006150.1 | + | 77909 | 0.67 | 0.830977 |
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Target: 5'- aAUCCGCCGGCUucuUGGaCcGCUuGCGCAa -3' miRNA: 3'- -UAGGCGGCCGA---GCCaGcUGAcUGUGU- -5' |
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| 29214 | 5' | -59.1 | NC_006150.1 | + | 118264 | 0.67 | 0.81463 |
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Target: 5'- -aCCGCgGGCUCGaGcggcUCGACUuugugGACGCGc -3' miRNA: 3'- uaGGCGgCCGAGC-C----AGCUGA-----CUGUGU- -5' |
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| 29214 | 5' | -59.1 | NC_006150.1 | + | 128753 | 0.68 | 0.780169 |
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Target: 5'- uUCCGCCGcGgUCGGUgGgAUUGACAa- -3' miRNA: 3'- uAGGCGGC-CgAGCCAgC-UGACUGUgu -5' |
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| 29214 | 5' | -59.1 | NC_006150.1 | + | 108467 | 0.7 | 0.627973 |
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Target: 5'- uUUCGCCgaGGCUCGGUCGGCgUGuuGCc -3' miRNA: 3'- uAGGCGG--CCGAGCCAGCUG-ACugUGu -5' |
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| 29214 | 5' | -59.1 | NC_006150.1 | + | 125903 | 1.06 | 0.003556 |
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Target: 5'- gAUCCGCCGGCUCGGUCGACUGACACAc -3' miRNA: 3'- -UAGGCGGCCGAGCCAGCUGACUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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