Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29215 | 3' | -50.9 | NC_006150.1 | + | 55407 | 0.66 | 0.99806 |
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Target: 5'- --aUCCACAUGCACg-----GCCGAGu -3' miRNA: 3'- cagAGGUGUACGUGaagaugCGGUUC- -5' |
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| 29215 | 3' | -50.9 | NC_006150.1 | + | 153256 | 0.66 | 0.995493 |
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Target: 5'- aGUCUCUACccguacgGCACUUCacugauucgcaggUAUGCCAGa -3' miRNA: 3'- -CAGAGGUGua-----CGUGAAG-------------AUGCGGUUc -5' |
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| 29215 | 3' | -50.9 | NC_006150.1 | + | 92006 | 0.67 | 0.994013 |
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Target: 5'- -gCUCCcaGCAUGCACgcgCUGCugGCCAu- -3' miRNA: 3'- caGAGG--UGUACGUGaa-GAUG--CGGUuc -5' |
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| 29215 | 3' | -50.9 | NC_006150.1 | + | 51577 | 0.67 | 0.994013 |
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Target: 5'- aUCUUCAC-UGCGCUUCa--GCCGAc -3' miRNA: 3'- cAGAGGUGuACGUGAAGaugCGGUUc -5' |
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| 29215 | 3' | -50.9 | NC_006150.1 | + | 220763 | 0.68 | 0.983044 |
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Target: 5'- -gCUCuCACAUGCGCgaCguugGCGCCAc- -3' miRNA: 3'- caGAG-GUGUACGUGaaGa---UGCGGUuc -5' |
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| 29215 | 3' | -50.9 | NC_006150.1 | + | 37051 | 0.69 | 0.980992 |
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Target: 5'- aUCUCaCGCcgGUGCGCagUCUGCGCgAGGc -3' miRNA: 3'- cAGAG-GUG--UACGUGa-AGAUGCGgUUC- -5' |
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| 29215 | 3' | -50.9 | NC_006150.1 | + | 138319 | 0.69 | 0.978525 |
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Target: 5'- uUCUCCACAccaucuUGCGgUUUUgguuccgcaucacGCGCCAGGa -3' miRNA: 3'- cAGAGGUGU------ACGUgAAGA-------------UGCGGUUC- -5' |
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| 29215 | 3' | -50.9 | NC_006150.1 | + | 70057 | 0.7 | 0.953502 |
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Target: 5'- ---gCCACGaGCGCUUCcagcUGCGCCGAGc -3' miRNA: 3'- cagaGGUGUaCGUGAAG----AUGCGGUUC- -5' |
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| 29215 | 3' | -50.9 | NC_006150.1 | + | 183886 | 0.75 | 0.806363 |
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Target: 5'- aGUCUCCAC-UGCGCcuccacCUGCGCCGGc -3' miRNA: 3'- -CAGAGGUGuACGUGaa----GAUGCGGUUc -5' |
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| 29215 | 3' | -50.9 | NC_006150.1 | + | 126744 | 1.11 | 0.009257 |
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Target: 5'- cGUCUCCACAUGCACUUCUACGCCAAGg -3' miRNA: 3'- -CAGAGGUGUACGUGAAGAUGCGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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