Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29218 | 3' | -57.3 | NC_006150.1 | + | 94590 | 0.66 | 0.911233 |
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Target: 5'- ---cGcGCgCGCCCUC-GUCGUAAGC-Ca -3' miRNA: 3'- uacaC-CG-GCGGGAGaCAGCAUUCGcG- -5' |
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| 29218 | 3' | -57.3 | NC_006150.1 | + | 152327 | 0.66 | 0.905283 |
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Target: 5'- -gGUGGCUGCUgUUgaucGUCGccacGAGCGCc -3' miRNA: 3'- uaCACCGGCGGgAGa---CAGCa---UUCGCG- -5' |
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| 29218 | 3' | -57.3 | NC_006150.1 | + | 39129 | 0.66 | 0.905283 |
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Target: 5'- -cGccGCCGCUCUCUuccGUCGaaccAAGCGCg -3' miRNA: 3'- uaCacCGGCGGGAGA---CAGCa---UUCGCG- -5' |
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| 29218 | 3' | -57.3 | NC_006150.1 | + | 205251 | 0.66 | 0.905283 |
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Target: 5'- ---aGGCCGUCCgc-GUCuUGGGCGCu -3' miRNA: 3'- uacaCCGGCGGGagaCAGcAUUCGCG- -5' |
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| 29218 | 3' | -57.3 | NC_006150.1 | + | 139663 | 0.67 | 0.872243 |
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Target: 5'- cUGaUGGCgGCaCaggCUGUCGU-AGCGCa -3' miRNA: 3'- uAC-ACCGgCG-Gga-GACAGCAuUCGCG- -5' |
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| 29218 | 3' | -57.3 | NC_006150.1 | + | 140378 | 0.67 | 0.872243 |
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Target: 5'- gGUGUGGa-GCgCCUCUGUgCGacaGAGCGUg -3' miRNA: 3'- -UACACCggCG-GGAGACA-GCa--UUCGCG- -5' |
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| 29218 | 3' | -57.3 | NC_006150.1 | + | 179765 | 0.68 | 0.825923 |
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Target: 5'- gAUGUGGCCcuccgGCaCCUCUGgag-AAGCGUu -3' miRNA: 3'- -UACACCGG-----CG-GGAGACagcaUUCGCG- -5' |
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| 29218 | 3' | -57.3 | NC_006150.1 | + | 27483 | 0.72 | 0.587979 |
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Target: 5'- -aGUGGCCGCCggauagCGUGAGCGUg -3' miRNA: 3'- uaCACCGGCGGgagacaGCAUUCGCG- -5' |
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| 29218 | 3' | -57.3 | NC_006150.1 | + | 88204 | 0.74 | 0.491262 |
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Target: 5'- cUG-GGCgGCCCUCUGggggCGUAGccauGCGCg -3' miRNA: 3'- uACaCCGgCGGGAGACa---GCAUU----CGCG- -5' |
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| 29218 | 3' | -57.3 | NC_006150.1 | + | 128761 | 1.09 | 0.002876 |
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Target: 5'- aAUGUGGCCGCCCUCUGUCGUAAGCGCu -3' miRNA: 3'- -UACACCGGCGGGAGACAGCAUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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