Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29221 | 5' | -59.2 | NC_006150.1 | + | 87564 | 0.66 | 0.842573 |
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Target: 5'- -aGGGGgGUUUGGGGGGUGagGGGUUc -3' miRNA: 3'- aaCCUUgCAGACUCCCCGCg-UCCGAc -5' |
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| 29221 | 5' | -59.2 | NC_006150.1 | + | 65462 | 0.66 | 0.801107 |
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Target: 5'- -aGGAACGUCUGcAGuuuGGCGCucaguucgggucGGGCUa -3' miRNA: 3'- aaCCUUGCAGAC-UCc--CCGCG------------UCCGAc -5' |
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| 29221 | 5' | -59.2 | NC_006150.1 | + | 135554 | 0.67 | 0.792351 |
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Target: 5'- gUGG-ACGUC-GAGGcGGUGCcAGGCg- -3' miRNA: 3'- aACCuUGCAGaCUCC-CCGCG-UCCGac -5' |
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| 29221 | 5' | -59.2 | NC_006150.1 | + | 183873 | 0.67 | 0.765295 |
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Target: 5'- -gGGAGCGguggUUGcAGcGGCGCAGGCa- -3' miRNA: 3'- aaCCUUGCa---GAC-UCcCCGCGUCCGac -5' |
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| 29221 | 5' | -59.2 | NC_006150.1 | + | 219788 | 0.71 | 0.521566 |
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Target: 5'- gUGGAACGUCcGAGgcGGGCGCGuuguGGCg- -3' miRNA: 3'- aACCUUGCAGaCUC--CCCGCGU----CCGac -5' |
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| 29221 | 5' | -59.2 | NC_006150.1 | + | 130673 | 1.05 | 0.00348 |
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Target: 5'- uUUGGAACGUCUGAGGGGCGCAGGCUGc -3' miRNA: 3'- -AACCUUGCAGACUCCCCGCGUCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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