Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29222 | 3' | -50.9 | NC_006150.1 | + | 217600 | 0.66 | 0.997181 |
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Target: 5'- gCAGGGCGguGCGcUCUUGGCgacugcgccaCCGCAGu -3' miRNA: 3'- -GUCCUGC--UGU-AGAACCGaa--------GGUGUUu -5' |
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| 29222 | 3' | -50.9 | NC_006150.1 | + | 99556 | 0.66 | 0.994683 |
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Target: 5'- cCAGGGCGugGUCUcuGCggCCGCu-- -3' miRNA: 3'- -GUCCUGCugUAGAacCGaaGGUGuuu -5' |
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| 29222 | 3' | -50.9 | NC_006150.1 | + | 206334 | 0.69 | 0.970077 |
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Target: 5'- -cGGACgGGCAUCU--GUUUCCACAGAu -3' miRNA: 3'- guCCUG-CUGUAGAacCGAAGGUGUUU- -5' |
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| 29222 | 3' | -50.9 | NC_006150.1 | + | 149256 | 0.69 | 0.966965 |
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Target: 5'- -cGGGCGACGagg-GGCUUCCACc-- -3' miRNA: 3'- guCCUGCUGUagaaCCGAAGGUGuuu -5' |
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| 29222 | 3' | -50.9 | NC_006150.1 | + | 133250 | 0.71 | 0.943445 |
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Target: 5'- gCAGGGCGAUAUgUgcaGGCUcgUCCGCGc- -3' miRNA: 3'- -GUCCUGCUGUAgAa--CCGA--AGGUGUuu -5' |
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| 29222 | 3' | -50.9 | NC_006150.1 | + | 66277 | 0.76 | 0.744814 |
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Target: 5'- aGGGACGuCAUCUUGGCUUgCCGUAAu -3' miRNA: 3'- gUCCUGCuGUAGAACCGAA-GGUGUUu -5' |
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| 29222 | 3' | -50.9 | NC_006150.1 | + | 132220 | 1.07 | 0.014376 |
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Target: 5'- aCAGGACGACAUCUUGGCUUCCACAAAg -3' miRNA: 3'- -GUCCUGCUGUAGAACCGAAGGUGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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