Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29228 | 3' | -57.1 | NC_006150.1 | + | 125465 | 0.66 | 0.933581 |
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Target: 5'- aCGCCGGCC-UGACGGUCg-CGCcGa -3' miRNA: 3'- cGUGGUCGGcACUGUCAGgaGCGaCa -5' |
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| 29228 | 3' | -57.1 | NC_006150.1 | + | 64173 | 0.67 | 0.873772 |
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Target: 5'- aGCGCCuGCUG-GACcuGUUCUUGCUGc -3' miRNA: 3'- -CGUGGuCGGCaCUGu-CAGGAGCGACa -5' |
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| 29228 | 3' | -57.1 | NC_006150.1 | + | 848 | 0.67 | 0.866611 |
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Target: 5'- gGCGCCGGCgGUguaGACGGUguggCCgcgagCGCUGa -3' miRNA: 3'- -CGUGGUCGgCA---CUGUCA----GGa----GCGACa -5' |
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| 29228 | 3' | -57.1 | NC_006150.1 | + | 82490 | 0.69 | 0.802668 |
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Target: 5'- uGCGCCAGaaCCGUacACAGaCUUCGCUGUu -3' miRNA: 3'- -CGUGGUC--GGCAc-UGUCaGGAGCGACA- -5' |
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| 29228 | 3' | -57.1 | NC_006150.1 | + | 140893 | 0.69 | 0.78508 |
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Target: 5'- gGCACCGGCCGUGcCGGUCaccagaaGCg-- -3' miRNA: 3'- -CGUGGUCGGCACuGUCAGgag----CGaca -5' |
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| 29228 | 3' | -57.1 | NC_006150.1 | + | 71553 | 0.7 | 0.766974 |
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Target: 5'- aCACCGGCUGUGcCGuGUCuCUCuGCUGUu -3' miRNA: 3'- cGUGGUCGGCACuGU-CAG-GAG-CGACA- -5' |
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| 29228 | 3' | -57.1 | NC_006150.1 | + | 116749 | 0.7 | 0.757747 |
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Target: 5'- cCACCGGCaCGaUGGCGGUUUUcCGCUGg -3' miRNA: 3'- cGUGGUCG-GC-ACUGUCAGGA-GCGACa -5' |
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| 29228 | 3' | -57.1 | NC_006150.1 | + | 149805 | 0.7 | 0.748415 |
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Target: 5'- -uGCCAGCCcaaGUGGCGGUgcgagCCUgGCUGg -3' miRNA: 3'- cgUGGUCGG---CACUGUCA-----GGAgCGACa -5' |
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| 29228 | 3' | -57.1 | NC_006150.1 | + | 36682 | 0.71 | 0.661105 |
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Target: 5'- uGCGCUAGCCGUggccacugGACAGUa--CGCUGUg -3' miRNA: 3'- -CGUGGUCGGCA--------CUGUCAggaGCGACA- -5' |
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| 29228 | 3' | -57.1 | NC_006150.1 | + | 142843 | 1.1 | 0.002988 |
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Target: 5'- aGCACCAGCCGUGACAGUCCUCGCUGUg -3' miRNA: 3'- -CGUGGUCGGCACUGUCAGGAGCGACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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