Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29229 | 3' | -59.7 | NC_006150.1 | + | 182654 | 0.66 | 0.809256 |
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Target: 5'- cCGGAGCC-CCCgCCGaGGCcucauucGCCa -3' miRNA: 3'- -GCCUCGGuGGGaGGC-CCGaaaa---CGGa -5' |
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| 29229 | 3' | -59.7 | NC_006150.1 | + | 103688 | 0.67 | 0.7825 |
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Target: 5'- aCGGAGCgcguccucgcaggCAUCCUCgCGGGCaugacgGCCg -3' miRNA: 3'- -GCCUCG-------------GUGGGAG-GCCCGaaaa--CGGa -5' |
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| 29229 | 3' | -59.7 | NC_006150.1 | + | 117643 | 0.68 | 0.719032 |
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Target: 5'- uGGGcGCCAauCCgUCCGGGaugUUGCCg -3' miRNA: 3'- gCCU-CGGU--GGgAGGCCCgaaAACGGa -5' |
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| 29229 | 3' | -59.7 | NC_006150.1 | + | 199705 | 0.68 | 0.699911 |
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Target: 5'- uGGuuaccAGCUACCUUCUGGGCgcgcGCCa -3' miRNA: 3'- gCC-----UCGGUGGGAGGCCCGaaaaCGGa -5' |
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| 29229 | 3' | -59.7 | NC_006150.1 | + | 143022 | 0.69 | 0.670859 |
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Target: 5'- gGGAGCCACCCUgUCuGGCU--UGCa- -3' miRNA: 3'- gCCUCGGUGGGA-GGcCCGAaaACGga -5' |
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| 29229 | 3' | -59.7 | NC_006150.1 | + | 125476 | 0.7 | 0.582916 |
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Target: 5'- aCGGucgcGCCgACCUUUCGGGUggUUUGCCa -3' miRNA: 3'- -GCCu---CGG-UGGGAGGCCCGa-AAACGGa -5' |
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| 29229 | 3' | -59.7 | NC_006150.1 | + | 23312 | 0.71 | 0.573222 |
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Target: 5'- aGGGGCCG-CCUCCaaGGGCgaugGCCa -3' miRNA: 3'- gCCUCGGUgGGAGG--CCCGaaaaCGGa -5' |
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| 29229 | 3' | -59.7 | NC_006150.1 | + | 88068 | 0.72 | 0.506729 |
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Target: 5'- gCGGAcugcGCCACCgagCUCCGGGCag--GCCg -3' miRNA: 3'- -GCCU----CGGUGG---GAGGCCCGaaaaCGGa -5' |
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| 29229 | 3' | -59.7 | NC_006150.1 | + | 143311 | 1.07 | 0.002776 |
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Target: 5'- uCGGAGCCACCCUCCGGGCUUUUGCCUc -3' miRNA: 3'- -GCCUCGGUGGGAGGCCCGAAAACGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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