Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29232 | 5' | -54.8 | NC_006150.1 | + | 183101 | 0.66 | 0.971282 |
Target: 5'- -gUGGCGaaaGUCg--GGGCCGGUggUCGAGu -3' miRNA: 3'- ugGCCGC---CAGaaaCCCGGCUA--AGUUC- -5' |
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29232 | 5' | -54.8 | NC_006150.1 | + | 55313 | 0.66 | 0.962001 |
Target: 5'- uACCGGUGGgacaagGGGCCuuUUUGAGa -3' miRNA: 3'- -UGGCCGCCagaaa-CCCGGcuAAGUUC- -5' |
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29232 | 5' | -54.8 | NC_006150.1 | + | 1976 | 0.68 | 0.932772 |
Target: 5'- gGCUgaGGCGGUCUgcGGuGCCGGUgcgCAu- -3' miRNA: 3'- -UGG--CCGCCAGAaaCC-CGGCUAa--GUuc -5' |
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29232 | 5' | -54.8 | NC_006150.1 | + | 137772 | 0.68 | 0.927686 |
Target: 5'- uUCGGCGGUUUggccUGGGCCu-UUgGAGg -3' miRNA: 3'- uGGCCGCCAGAa---ACCCGGcuAAgUUC- -5' |
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29232 | 5' | -54.8 | NC_006150.1 | + | 148584 | 1.09 | 0.005519 |
Target: 5'- gACCGGCGGUCUUUGGGCCGAUUCAAGa -3' miRNA: 3'- -UGGCCGCCAGAAACCCGGCUAAGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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