Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29233 | 5' | -51.7 | NC_006150.1 | + | 146650 | 0.66 | 0.995974 |
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Target: 5'- gGUCGCCcgCAacUCCUUGAAgauGCCUGcCAa -3' miRNA: 3'- gCAGUGGa-GU--AGGAAUUU---CGGACcGU- -5' |
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| 29233 | 5' | -51.7 | NC_006150.1 | + | 105692 | 0.66 | 0.993704 |
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Target: 5'- ---gGCCUCAUCCgucGGGCUggacaUGGCAg -3' miRNA: 3'- gcagUGGAGUAGGaauUUCGG-----ACCGU- -5' |
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| 29233 | 5' | -51.7 | NC_006150.1 | + | 199585 | 0.66 | 0.993704 |
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Target: 5'- uCGUCACCgccuaCAUCau--GAGCgUGGCu -3' miRNA: 3'- -GCAGUGGa----GUAGgaauUUCGgACCGu -5' |
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| 29233 | 5' | -51.7 | NC_006150.1 | + | 37247 | 0.66 | 0.992751 |
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Target: 5'- aG-CGCCUUuuUUUUUGuGGCCUGGCAg -3' miRNA: 3'- gCaGUGGAGu-AGGAAUuUCGGACCGU- -5' |
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| 29233 | 5' | -51.7 | NC_006150.1 | + | 136292 | 0.67 | 0.98774 |
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Target: 5'- uGUCGCCaUGUCCUUucGGgUUGGCGc -3' miRNA: 3'- gCAGUGGaGUAGGAAuuUCgGACCGU- -5' |
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| 29233 | 5' | -51.7 | NC_006150.1 | + | 158108 | 0.68 | 0.980395 |
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Target: 5'- gGUCcagcaccuuCCUCuUCCUUAAuaugaGGCUUGGCAu -3' miRNA: 3'- gCAGu--------GGAGuAGGAAUU-----UCGGACCGU- -5' |
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| 29233 | 5' | -51.7 | NC_006150.1 | + | 80291 | 0.72 | 0.879313 |
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Target: 5'- -uUCugCUCAUCC----GGCCUGGCc -3' miRNA: 3'- gcAGugGAGUAGGaauuUCGGACCGu -5' |
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| 29233 | 5' | -51.7 | NC_006150.1 | + | 2437 | 0.75 | 0.778552 |
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Target: 5'- aG-CACCUCGUUCUgcucguucGGCCUGGCGg -3' miRNA: 3'- gCaGUGGAGUAGGAauu-----UCGGACCGU- -5' |
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| 29233 | 5' | -51.7 | NC_006150.1 | + | 149917 | 1.09 | 0.009645 |
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Target: 5'- cCGUCACCUCAUCCUUAAAGCCUGGCAc -3' miRNA: 3'- -GCAGUGGAGUAGGAAUUUCGGACCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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