Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29235 | 3' | -45.6 | NC_006150.1 | + | 148773 | 0.66 | 1 |
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Target: 5'- aGC-UGAUGCUGg--GGUCaGGgcGUCCa -3' miRNA: 3'- -CGaACUAUGGCaugCUAG-CCauUAGG- -5' |
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| 29235 | 3' | -45.6 | NC_006150.1 | + | 140981 | 0.66 | 1 |
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Target: 5'- cGCUgaGGUuCUGggcgGCGAUuaCGGUGGUCCc -3' miRNA: 3'- -CGAa-CUAuGGCa---UGCUA--GCCAUUAGG- -5' |
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| 29235 | 3' | -45.6 | NC_006150.1 | + | 106618 | 0.67 | 0.999999 |
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Target: 5'- cGCUguUGcccaacagGCCGUACGAaUCGGacugaaaugGAUCCg -3' miRNA: 3'- -CGA--ACua------UGGCAUGCU-AGCCa--------UUAGG- -5' |
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| 29235 | 3' | -45.6 | NC_006150.1 | + | 207537 | 0.67 | 0.999997 |
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Target: 5'- cGCUUGcucuUGCUGgGCGGUagaaGGUcAUCCa -3' miRNA: 3'- -CGAACu---AUGGCaUGCUAg---CCAuUAGG- -5' |
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| 29235 | 3' | -45.6 | NC_006150.1 | + | 82341 | 0.67 | 0.999997 |
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Target: 5'- cGCUUaGAU-CgGUGCGAUCGGca--CCa -3' miRNA: 3'- -CGAA-CUAuGgCAUGCUAGCCauuaGG- -5' |
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| 29235 | 3' | -45.6 | NC_006150.1 | + | 116554 | 0.68 | 0.999992 |
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Target: 5'- cGCUUGAUAUCGUcguugagugcagcGCaGAUCGuagGAUCUg -3' miRNA: 3'- -CGAACUAUGGCA-------------UG-CUAGCca-UUAGG- -5' |
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| 29235 | 3' | -45.6 | NC_006150.1 | + | 64552 | 0.68 | 0.999989 |
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Target: 5'- cGCUUGuc-CCGUGauua-GGUAAUCCa -3' miRNA: 3'- -CGAACuauGGCAUgcuagCCAUUAGG- -5' |
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| 29235 | 3' | -45.6 | NC_006150.1 | + | 128934 | 0.73 | 0.998051 |
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Target: 5'- gGCUgUGucuCCGUACGAUCGGU--UUCg -3' miRNA: 3'- -CGA-ACuauGGCAUGCUAGCCAuuAGG- -5' |
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| 29235 | 3' | -45.6 | NC_006150.1 | + | 152587 | 1.16 | 0.022784 |
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Target: 5'- uGCUUGAUACCGUACGAUCGGUAAUCCc -3' miRNA: 3'- -CGAACUAUGGCAUGCUAGCCAUUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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