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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29242 | 5' | -46 | NC_006150.1 | + | 198117 | 0.66 | 0.999997 |
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Target: 5'- -----gACCGCCaCGUUaaGCGUGUGUGc -3' miRNA: 3'- uaauuaUGGCGG-GUAAc-UGCACAUAU- -5' |
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| 29242 | 5' | -46 | NC_006150.1 | + | 207779 | 0.66 | 0.999992 |
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Target: 5'- ----cUACCGCCCAgcaagagcaaGCGUGUAg- -3' miRNA: 3'- uaauuAUGGCGGGUaac-------UGCACAUau -5' |
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| 29242 | 5' | -46 | NC_006150.1 | + | 120905 | 0.68 | 0.999808 |
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Target: 5'- -----aGCCGCCUugucggGACGUGUAg- -3' miRNA: 3'- uaauuaUGGCGGGuaa---CUGCACAUau -5' |
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| 29242 | 5' | -46 | NC_006150.1 | + | 179780 | 0.68 | 0.999808 |
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Target: 5'- cUUAAUACUGCUCAU-GAUGUGg--- -3' miRNA: 3'- uAAUUAUGGCGGGUAaCUGCACauau -5' |
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| 29242 | 5' | -46 | NC_006150.1 | + | 161050 | 0.69 | 0.999753 |
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Target: 5'- -aUAggACCGcCCCAUUGACGUcaAUGg -3' miRNA: 3'- uaAUuaUGGC-GGGUAACUGCAcaUAU- -5' |
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| 29242 | 5' | -46 | NC_006150.1 | + | 161307 | 0.7 | 0.999043 |
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Target: 5'- --aAGUACaUGCCCAUUGACGUcaAUGg -3' miRNA: 3'- uaaUUAUG-GCGGGUAACUGCAcaUAU- -5' |
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| 29242 | 5' | -46 | NC_006150.1 | + | 161630 | 0.76 | 0.942235 |
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Target: 5'- -----gACCGCCCAUUGACGUa---- -3' miRNA: 3'- uaauuaUGGCGGGUAACUGCAcauau -5' |
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| 29242 | 5' | -46 | NC_006150.1 | + | 161567 | 1.06 | 0.048946 |
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Target: 5'- cAUUAAUACCGCCCAUUGACGUGUAUAg -3' miRNA: 3'- -UAAUUAUGGCGGGUAACUGCACAUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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