miRNA: 3'- cauuaGUCa--GGUUGUGGU-UCGGCGGa -5'
miRNA: 3'- -CAUuaGUCaGGUUgUGGU-UCGGCGGA- -5'
miRNA: 3'- cauUAGUCAGG-UUGugGUU------CGGCGGa -5'
miRNA: 3'- -CAUUAGUCAGGUUGUGGUUCGGCGGA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29244 | 3' | -51.9 | NC_006150.1 | + | 65174 | 0.71 | 0.903008 |
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Target: 5'- ----aCGGcccCCAGCGCCAcGGCCGCCg -3' miRNA: 3'- cauuaGUCa--GGUUGUGGU-UCGGCGGa -5' |
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| 29244 | 3' | -51.9 | NC_006150.1 | + | 119114 | 0.71 | 0.896494 |
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Target: 5'- cGUGcgCAG-CCGAgGCCAgGGCCGUCUg -3' miRNA: 3'- -CAUuaGUCaGGUUgUGGU-UCGGCGGA- -5' |
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| 29244 | 3' | -51.9 | NC_006150.1 | + | 184848 | 0.72 | 0.860441 |
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Target: 5'- --uGUCAGUCCgAGCugCAGcgcaacGCCGCCa -3' miRNA: 3'- cauUAGUCAGG-UUGugGUU------CGGCGGa -5' |
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| 29244 | 3' | -51.9 | NC_006150.1 | + | 169641 | 1.08 | 0.011119 |
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Target: 5'- aGUAAUCAGUCCAACACCAAGCCGCCUu -3' miRNA: 3'- -CAUUAGUCAGGUUGUGGUUCGGCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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