Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2925 | 5' | -59.2 | NC_001493.1 | + | 105899 | 0.68 | 0.602859 |
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Target: 5'- gGGGGUGaggguuaucaguuucUUCGCGGugugaucaucgcCCAuGUCUGCGCGCg -3' miRNA: 3'- -CCCCGC---------------AGGCGCC------------GGU-UAGACGUGUG- -5' |
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| 2925 | 5' | -59.2 | NC_001493.1 | + | 106768 | 0.68 | 0.598827 |
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Target: 5'- gGGGGCGaCuCGUGGCUAAaCUGgGCGa -3' miRNA: 3'- -CCCCGCaG-GCGCCGGUUaGACgUGUg -5' |
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| 2925 | 5' | -59.2 | NC_001493.1 | + | 32607 | 0.69 | 0.577736 |
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Target: 5'- cGGGCGuuuacggUCCGgGGCCAuAUCcGCGCGu -3' miRNA: 3'- cCCCGC-------AGGCgCCGGU-UAGaCGUGUg -5' |
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| 2925 | 5' | -59.2 | NC_001493.1 | + | 32798 | 0.73 | 0.368307 |
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Target: 5'- aGGGuaGUCCGCGaCCGggAUCgGCACGCg -3' miRNA: 3'- -CCCcgCAGGCGCcGGU--UAGaCGUGUG- -5' |
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| 2925 | 5' | -59.2 | NC_001493.1 | + | 43458 | 0.76 | 0.24425 |
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Target: 5'- cGGGGgGUCgaucugCGCGGCCGAUCUcGUAgACg -3' miRNA: 3'- -CCCCgCAG------GCGCCGGUUAGA-CGUgUG- -5' |
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| 2925 | 5' | -59.2 | NC_001493.1 | + | 53958 | 1 | 0.005787 |
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Target: 5'- uGGGGCGUCCGCGGCCAAUCcGUACACu -3' miRNA: 3'- -CCCCGCAGGCGCCGGUUAGaCGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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