Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29251 | 5' | -50.6 | NC_006150.1 | + | 160682 | 0.66 | 0.998055 |
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Target: 5'- -aUCaAUAGCACCGccCCUUaUGGGGGu -3' miRNA: 3'- ucAGaUAUCGUGGUucGGAA-ACCCUC- -5' |
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| 29251 | 5' | -50.6 | NC_006150.1 | + | 65650 | 0.66 | 0.997682 |
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Target: 5'- uGUCUcgGGCucCCGAGCCcaa-GGAGg -3' miRNA: 3'- uCAGAuaUCGu-GGUUCGGaaacCCUC- -5' |
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| 29251 | 5' | -50.6 | NC_006150.1 | + | 44654 | 0.66 | 0.996753 |
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Target: 5'- uGUCUAaAGUggGCCAAuGCUUUcGGGGGg -3' miRNA: 3'- uCAGAUaUCG--UGGUU-CGGAAaCCCUC- -5' |
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| 29251 | 5' | -50.6 | NC_006150.1 | + | 171231 | 0.66 | 0.996753 |
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Target: 5'- uGUCUGUGGCGCgGcGGCC--UGGGu- -3' miRNA: 3'- uCAGAUAUCGUGgU-UCGGaaACCCuc -5' |
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| 29251 | 5' | -50.6 | NC_006150.1 | + | 170680 | 0.66 | 0.996184 |
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Target: 5'- -aUCUGUuGCACUAAGCCUU--GGAa -3' miRNA: 3'- ucAGAUAuCGUGGUUCGGAAacCCUc -5' |
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| 29251 | 5' | -50.6 | NC_006150.1 | + | 185261 | 0.67 | 0.995535 |
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Target: 5'- -aUCg--GGCACUAAGCCUagUGGGu- -3' miRNA: 3'- ucAGauaUCGUGGUUCGGAa-ACCCuc -5' |
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| 29251 | 5' | -50.6 | NC_006150.1 | + | 59465 | 0.68 | 0.990824 |
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Target: 5'- uGUCUAUGGCuACCAAGUgucagCUggaGGGAa -3' miRNA: 3'- uCAGAUAUCG-UGGUUCG-----GAaa-CCCUc -5' |
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| 29251 | 5' | -50.6 | NC_006150.1 | + | 168340 | 0.7 | 0.964184 |
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Target: 5'- cAGUCUcgGGCugCAucauCCUcUGGGAGc -3' miRNA: 3'- -UCAGAuaUCGugGUuc--GGAaACCCUC- -5' |
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| 29251 | 5' | -50.6 | NC_006150.1 | + | 161938 | 0.71 | 0.952918 |
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Target: 5'- uGGUCgacuUGGCACCGuGCCaagUUUGGGGu -3' miRNA: 3'- -UCAGau--AUCGUGGUuCGG---AAACCCUc -5' |
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| 29251 | 5' | -50.6 | NC_006150.1 | + | 186337 | 0.8 | 0.559965 |
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Target: 5'- -aUCUAUGGCGCCuGGCCUcUGGGGa -3' miRNA: 3'- ucAGAUAUCGUGGuUCGGAaACCCUc -5' |
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| 29251 | 5' | -50.6 | NC_006150.1 | + | 186546 | 1.01 | 0.034799 |
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Target: 5'- aAGUCUAUAGCACCAAGCCUUUGGaGAGu -3' miRNA: 3'- -UCAGAUAUCGUGGUUCGGAAACC-CUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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