Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29252 | 3' | -48.9 | NC_006150.1 | + | 132729 | 0.66 | 0.999626 |
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Target: 5'- uCGUGAGAAUaucacgaagcaGC-UCGUCCUGG-GCc- -3' miRNA: 3'- -GUAUUCUUA-----------CGaAGCAGGACCaCGac -5' |
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| 29252 | 3' | -48.9 | NC_006150.1 | + | 152033 | 0.67 | 0.999116 |
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Target: 5'- gGUGGGggUGCUgguaUUGcCUgcggUGGUGCUGc -3' miRNA: 3'- gUAUUCuuACGA----AGCaGG----ACCACGAC- -5' |
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| 29252 | 3' | -48.9 | NC_006150.1 | + | 12942 | 0.68 | 0.996047 |
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Target: 5'- gCGUGGGggUGCUgacucgaccggcggUCGUUC-GGUGCg- -3' miRNA: 3'- -GUAUUCuuACGA--------------AGCAGGaCCACGac -5' |
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| 29252 | 3' | -48.9 | NC_006150.1 | + | 196280 | 0.69 | 0.993077 |
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Target: 5'- uGUuGGAcggGUUUCGUUCUgGGUGCUGg -3' miRNA: 3'- gUAuUCUua-CGAAGCAGGA-CCACGAC- -5' |
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| 29252 | 3' | -48.9 | NC_006150.1 | + | 213144 | 0.69 | 0.992025 |
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Target: 5'- --cAGGAGUGCUU-GUCUucguugucaucgUGGUGCUGc -3' miRNA: 3'- guaUUCUUACGAAgCAGG------------ACCACGAC- -5' |
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| 29252 | 3' | -48.9 | NC_006150.1 | + | 147775 | 0.71 | 0.973231 |
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Target: 5'- -uUGAGAGUGCcacgcUCGUCC-GGUGcCUGg -3' miRNA: 3'- guAUUCUUACGa----AGCAGGaCCAC-GAC- -5' |
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| 29252 | 3' | -48.9 | NC_006150.1 | + | 53187 | 0.71 | 0.973231 |
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Target: 5'- ---uGGggUGUUUUGUCUUGGgGCUGu -3' miRNA: 3'- guauUCuuACGAAGCAGGACCaCGAC- -5' |
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| 29252 | 3' | -48.9 | NC_006150.1 | + | 190829 | 0.76 | 0.869063 |
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Target: 5'- --------aGCUUgGUCCUGGUGCUGu -3' miRNA: 3'- guauucuuaCGAAgCAGGACCACGAC- -5' |
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| 29252 | 3' | -48.9 | NC_006150.1 | + | 191104 | 1.09 | 0.017748 |
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Target: 5'- aCAUAAGAAUGCUUCGUCCUGGUGCUGu -3' miRNA: 3'- -GUAUUCUUACGAAGCAGGACCACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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