Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29255 | 5' | -52.6 | NC_006150.1 | + | 173648 | 0.66 | 0.989808 |
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Target: 5'- gUCGGCCUUGAGcugcUGCaaaUCCACUg -3' miRNA: 3'- aAGUUGGGACUCcuaaACGg--AGGUGG- -5' |
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| 29255 | 5' | -52.6 | NC_006150.1 | + | 130774 | 0.67 | 0.981396 |
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Target: 5'- gUUCAGCCCcGAGcgccUUUGCgCUCCAUa -3' miRNA: 3'- -AAGUUGGGaCUCcu--AAACG-GAGGUGg -5' |
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| 29255 | 5' | -52.6 | NC_006150.1 | + | 122027 | 0.68 | 0.965455 |
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Target: 5'- cUCaAACCCUGAaGAgugGCCaacacgUCCACCa -3' miRNA: 3'- aAG-UUGGGACUcCUaaaCGG------AGGUGG- -5' |
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| 29255 | 5' | -52.6 | NC_006150.1 | + | 45868 | 0.68 | 0.962075 |
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Target: 5'- -gUAugCCUagcGuGGAUUUGUCUCCGCa -3' miRNA: 3'- aaGUugGGA---CuCCUAAACGGAGGUGg -5' |
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| 29255 | 5' | -52.6 | NC_006150.1 | + | 148708 | 0.69 | 0.950575 |
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Target: 5'- ---cGCCCUGAGGAgcUUGgCUCCAg- -3' miRNA: 3'- aaguUGGGACUCCUa-AACgGAGGUgg -5' |
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| 29255 | 5' | -52.6 | NC_006150.1 | + | 123511 | 0.69 | 0.936954 |
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Target: 5'- -gCGGCCUgguuGGAgUUGCCUuCCACCg -3' miRNA: 3'- aaGUUGGGacu-CCUaAACGGA-GGUGG- -5' |
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| 29255 | 5' | -52.6 | NC_006150.1 | + | 221409 | 0.7 | 0.926662 |
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Target: 5'- aUUCAgcGCCCUGGGGAggaaagcUGCCU--GCCg -3' miRNA: 3'- -AAGU--UGGGACUCCUaa-----ACGGAggUGG- -5' |
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| 29255 | 5' | -52.6 | NC_006150.1 | + | 75982 | 0.7 | 0.903151 |
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Target: 5'- --uGGCgCCUGGGGGcgaGUCUCCGCCg -3' miRNA: 3'- aagUUG-GGACUCCUaaaCGGAGGUGG- -5' |
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| 29255 | 5' | -52.6 | NC_006150.1 | + | 85932 | 0.75 | 0.674834 |
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Target: 5'- -aCGugCCUGAGGAgg-GCCgagcgcCCACCg -3' miRNA: 3'- aaGUugGGACUCCUaaaCGGa-----GGUGG- -5' |
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| 29255 | 5' | -52.6 | NC_006150.1 | + | 203298 | 1.11 | 0.00615 |
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Target: 5'- cUUCAACCCUGAGGAUUUGCCUCCACCa -3' miRNA: 3'- -AAGUUGGGACUCCUAAACGGAGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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