Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29256 | 3' | -52.2 | NC_006150.1 | + | 70436 | 0.66 | 0.993195 |
Target: 5'- gGACCuGCGGCUUccugu-GCUCAACc -3' miRNA: 3'- gCUGGuCGUCGAGcauguuCGAGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 203699 | 0.66 | 0.993195 |
Target: 5'- uGGCCAGUAGCUuuagUGUAUcAGCgUGACg -3' miRNA: 3'- gCUGGUCGUCGA----GCAUGuUCGaGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 216646 | 0.66 | 0.992184 |
Target: 5'- uGACUGGUuGCUCGccguggcggcUGCGGuGCUCGGCa -3' miRNA: 3'- gCUGGUCGuCGAGC----------AUGUU-CGAGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 170031 | 0.66 | 0.992184 |
Target: 5'- gCGGCUcGCGGCgccgauaccggCGUGCAGGCuUUGACu -3' miRNA: 3'- -GCUGGuCGUCGa----------GCAUGUUCG-AGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 32957 | 0.66 | 0.992184 |
Target: 5'- cCGcCCGGCuccCUCGUGCGAGC--GACg -3' miRNA: 3'- -GCuGGUCGuc-GAGCAUGUUCGagUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 31857 | 0.66 | 0.991058 |
Target: 5'- -uGCCAGCuGGCUCcuuucccucUugGGGCUCGGCa -3' miRNA: 3'- gcUGGUCG-UCGAGc--------AugUUCGAGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 70087 | 0.67 | 0.988425 |
Target: 5'- gGACUGGCGGUggcgcugCGUGgAGGCUUGAUu -3' miRNA: 3'- gCUGGUCGUCGa------GCAUgUUCGAGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 107651 | 0.67 | 0.988425 |
Target: 5'- gCGAUCAGCGGaCUUGcUACuguGAGC-CGACg -3' miRNA: 3'- -GCUGGUCGUC-GAGC-AUG---UUCGaGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 152294 | 0.67 | 0.988425 |
Target: 5'- aGGCCAGCAGCaccaccGCAGGCaauacCAGCa -3' miRNA: 3'- gCUGGUCGUCGagca--UGUUCGa----GUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 122394 | 0.67 | 0.986901 |
Target: 5'- uCGuCCAGCGGCgCGUcACcguGGCUCGGg -3' miRNA: 3'- -GCuGGUCGUCGaGCA-UGu--UCGAGUUg -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 154826 | 0.67 | 0.985227 |
Target: 5'- uGGCCcucGCGGCcgcCGUGCucaucGCUCAGCa -3' miRNA: 3'- gCUGGu--CGUCGa--GCAUGuu---CGAGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 114753 | 0.67 | 0.983395 |
Target: 5'- uGAUuaaCGGCGGCuuugccucuUCGUACGAGCggucCAGCg -3' miRNA: 3'- gCUG---GUCGUCG---------AGCAUGUUCGa---GUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 117365 | 0.67 | 0.981396 |
Target: 5'- cCGACCGGCAGCc-------GCUCAACa -3' miRNA: 3'- -GCUGGUCGUCGagcauguuCGAGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 76468 | 0.68 | 0.974316 |
Target: 5'- aGACUAGUu-CUCccaauUACAAGCUCAACa -3' miRNA: 3'- gCUGGUCGucGAGc----AUGUUCGAGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 114304 | 0.68 | 0.974316 |
Target: 5'- aGACUgauGGCAGCU-GUGgGacGGCUCAGCc -3' miRNA: 3'- gCUGG---UCGUCGAgCAUgU--UCGAGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 72259 | 0.68 | 0.974316 |
Target: 5'- cCGACCAGUuGUUCG-GCGGcCUCGGCa -3' miRNA: 3'- -GCUGGUCGuCGAGCaUGUUcGAGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 170257 | 0.68 | 0.973513 |
Target: 5'- gCGACC-GUGGCUCGUGCucaccgccgaauguGAGCgCGGCc -3' miRNA: 3'- -GCUGGuCGUCGAGCAUG--------------UUCGaGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 124060 | 0.68 | 0.971569 |
Target: 5'- gGACCAG-AGC-CGUgACGAGUUUGACa -3' miRNA: 3'- gCUGGUCgUCGaGCA-UGUUCGAGUUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 181846 | 0.68 | 0.967692 |
Target: 5'- cCGugCAGCGuuuGCUuccuauguccgcaaCGUACAGGCUguGCa -3' miRNA: 3'- -GCugGUCGU---CGA--------------GCAUGUUCGAguUG- -5' |
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29256 | 3' | -52.2 | NC_006150.1 | + | 126220 | 0.7 | 0.941735 |
Target: 5'- aGACguGCGGUcgucuUCGUGCGGGCcCGAUg -3' miRNA: 3'- gCUGguCGUCG-----AGCAUGUUCGaGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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