Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29256 | 5' | -49.1 | NC_006150.1 | + | 130273 | 0.66 | 0.999562 |
Target: 5'- ----gGAUCUGCUGgagGACGAcgauACCGa- -3' miRNA: 3'- uuugaCUAGACGACa--UUGCU----UGGCga -5' |
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29256 | 5' | -49.1 | NC_006150.1 | + | 171820 | 0.66 | 0.999323 |
Target: 5'- -uGCUGG-CUGCUGUcgcucACGGGCUGUg -3' miRNA: 3'- uuUGACUaGACGACAu----UGCUUGGCGa -5' |
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29256 | 5' | -49.1 | NC_006150.1 | + | 114307 | 0.67 | 0.99898 |
Target: 5'- ----cGAcUCUGCUGcagcuCGAGCCGCUc -3' miRNA: 3'- uuugaCU-AGACGACauu--GCUUGGCGA- -5' |
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29256 | 5' | -49.1 | NC_006150.1 | + | 1978 | 0.67 | 0.99819 |
Target: 5'- uGGGCUGAggcggUCUGCgGUGcCGGugCGCa -3' miRNA: 3'- -UUUGACU-----AGACGaCAUuGCUugGCGa -5' |
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29256 | 5' | -49.1 | NC_006150.1 | + | 194978 | 0.68 | 0.997418 |
Target: 5'- --uCUGGUCUGCUcgAugGGACgGCa -3' miRNA: 3'- uuuGACUAGACGAcaUugCUUGgCGa -5' |
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29256 | 5' | -49.1 | NC_006150.1 | + | 178759 | 0.68 | 0.996389 |
Target: 5'- -cACUGAUggUGUUGUAACGGGgCGCg -3' miRNA: 3'- uuUGACUAg-ACGACAUUGCUUgGCGa -5' |
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29256 | 5' | -49.1 | NC_006150.1 | + | 180155 | 0.68 | 0.995045 |
Target: 5'- cAGAC-GAUCgcaccgccgugGCUGU-GCGAACCGCa -3' miRNA: 3'- -UUUGaCUAGa----------CGACAuUGCUUGGCGa -5' |
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29256 | 5' | -49.1 | NC_006150.1 | + | 135870 | 0.7 | 0.986876 |
Target: 5'- cGAGCUGAUCccuUGCUGgcaGCGGAuCUGCg -3' miRNA: 3'- -UUUGACUAG---ACGACau-UGCUU-GGCGa -5' |
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29256 | 5' | -49.1 | NC_006150.1 | + | 128711 | 0.75 | 0.870063 |
Target: 5'- gGGACUGGcacaCUGCUGUAACGAGgCGUUc -3' miRNA: 3'- -UUUGACUa---GACGACAUUGCUUgGCGA- -5' |
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29256 | 5' | -49.1 | NC_006150.1 | + | 205802 | 1.07 | 0.022653 |
Target: 5'- cAAACUGAUCUGCUGUAACGAACCGCUg -3' miRNA: 3'- -UUUGACUAGACGACAUUGCUUGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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