Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29257 | 3' | -58 | NC_006150.1 | + | 137232 | 0.66 | 0.903476 |
Target: 5'- -cGUGUGUgCgGACCGUGGUcgagUGUUa- -3' miRNA: 3'- cuCACACGgGgCUGGCACCG----ACAAca -5' |
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29257 | 3' | -58 | NC_006150.1 | + | 219877 | 0.7 | 0.705953 |
Target: 5'- cGAGUGUGuUCCCGACUGgGGUUGcgGg -3' miRNA: 3'- -CUCACAC-GGGGCUGGCaCCGACaaCa -5' |
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29257 | 3' | -58 | NC_006150.1 | + | 101286 | 0.71 | 0.637046 |
Target: 5'- -uGUGUGUCCUGGCaguGUGGCUGcUGg -3' miRNA: 3'- cuCACACGGGGCUGg--CACCGACaACa -5' |
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29257 | 3' | -58 | NC_006150.1 | + | 214766 | 1.06 | 0.00413 |
Target: 5'- gGAGUGUGCCCCGACCGUGGCUGUUGUc -3' miRNA: 3'- -CUCACACGGGGCUGGCACCGACAACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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