Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29257 | 5' | -60.3 | NC_006150.1 | + | 88598 | 0.71 | 0.566829 |
Target: 5'- gCACAgCUCGCaGGUgGGGGCGUcgcgaggCCCa -3' miRNA: 3'- aGUGU-GAGUG-CCGgCCCCGCGua-----GGG- -5' |
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29257 | 5' | -60.3 | NC_006150.1 | + | 132797 | 0.75 | 0.323653 |
Target: 5'- -aACGCUCuCGaGCgGGGGCGUcgCCCg -3' miRNA: 3'- agUGUGAGuGC-CGgCCCCGCGuaGGG- -5' |
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29257 | 5' | -60.3 | NC_006150.1 | + | 214729 | 1.12 | 0.00122 |
Target: 5'- gUCACACUCACGGCCGGGGCGCAUCCCa -3' miRNA: 3'- -AGUGUGAGUGCCGGCCCCGCGUAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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