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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29259 | 3' | -55.8 | NC_006150.1 | + | 82139 | 0.66 | 0.950819 |
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Target: 5'- cUCCgC-CCGGUCCUGccUCCugGAGUACg -3' miRNA: 3'- aAGG-GaGGCUAGGACucAGG--CUCAUG- -5' |
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| 29259 | 3' | -55.8 | NC_006150.1 | + | 146721 | 0.67 | 0.932772 |
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Target: 5'- -cUCCUCCGAUCCgGAcugacaUCCGAGaACu -3' miRNA: 3'- aaGGGAGGCUAGGaCUc-----AGGCUCaUG- -5' |
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| 29259 | 3' | -55.8 | NC_006150.1 | + | 34713 | 0.68 | 0.871635 |
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Target: 5'- -gCUCUCCGAacgacuuccccUUCUGAGUCCucuucgcugaGAGUGCu -3' miRNA: 3'- aaGGGAGGCU-----------AGGACUCAGG----------CUCAUG- -5' |
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| 29259 | 3' | -55.8 | NC_006150.1 | + | 217592 | 0.68 | 0.864315 |
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Target: 5'- aUUCCCgugCCG--CCUGAGUCUGAGc-- -3' miRNA: 3'- -AAGGGa--GGCuaGGACUCAGGCUCaug -5' |
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| 29259 | 3' | -55.8 | NC_006150.1 | + | 167812 | 0.69 | 0.840359 |
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Target: 5'- aUUCCCUCCaaGAUCCUgaaaaaacuugaaGAGUcggacuggauaCCGGGUGCc -3' miRNA: 3'- -AAGGGAGG--CUAGGA-------------CUCA-----------GGCUCAUG- -5' |
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| 29259 | 3' | -55.8 | NC_006150.1 | + | 217877 | 1.08 | 0.004948 |
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Target: 5'- gUUCCCUCCGAUCCUGAGUCCGAGUACg -3' miRNA: 3'- -AAGGGAGGCUAGGACUCAGGCUCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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