Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2927 | 5' | -47.8 | NC_001493.1 | + | 13313 | 0.66 | 0.998793 |
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Target: 5'- gGAGACUCGGggaacugcgggcCGUgcggugCUCGGcGGGACGUg -3' miRNA: 3'- -UUUUGAGUU------------GUAa-----GAGUCuCCCUGCA- -5' |
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| 2927 | 5' | -47.8 | NC_001493.1 | + | 128867 | 0.66 | 0.998793 |
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Target: 5'- gGAGACUCGGggaacugcgggcCGUgcggugCUCGGcGGGACGUg -3' miRNA: 3'- -UUUUGAGUU------------GUAa-----GAGUCuCCCUGCA- -5' |
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| 2927 | 5' | -47.8 | NC_001493.1 | + | 38875 | 0.66 | 0.998526 |
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Target: 5'- cAAGCUCAuACAggugCGGGGGGACa- -3' miRNA: 3'- uUUUGAGU-UGUaagaGUCUCCCUGca -5' |
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| 2927 | 5' | -47.8 | NC_001493.1 | + | 25467 | 0.68 | 0.994045 |
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Target: 5'- --uACUCAACAcggUCUcCGGGGGGAUc- -3' miRNA: 3'- uuuUGAGUUGUa--AGA-GUCUCCCUGca -5' |
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| 2927 | 5' | -47.8 | NC_001493.1 | + | 112812 | 0.68 | 0.993066 |
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Target: 5'- cAGGCUCGACGcgaacUUCUgGguGAGGGACGc -3' miRNA: 3'- uUUUGAGUUGU-----AAGAgU--CUCCCUGCa -5' |
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| 2927 | 5' | -47.8 | NC_001493.1 | + | 14190 | 0.71 | 0.968663 |
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Target: 5'- ---cCUCAACGagCUCGcGGGGGACGa -3' miRNA: 3'- uuuuGAGUUGUaaGAGU-CUCCCUGCa -5' |
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| 2927 | 5' | -47.8 | NC_001493.1 | + | 129744 | 0.71 | 0.968663 |
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Target: 5'- ---cCUCAACGagCUCGcGGGGGACGa -3' miRNA: 3'- uuuuGAGUUGUaaGAGU-CUCCCUGCa -5' |
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| 2927 | 5' | -47.8 | NC_001493.1 | + | 134017 | 0.75 | 0.858227 |
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Target: 5'- gAGAACgCGACGgauuggCUCAGAGGGAUGa -3' miRNA: 3'- -UUUUGaGUUGUaa----GAGUCUCCCUGCa -5' |
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| 2927 | 5' | -47.8 | NC_001493.1 | + | 18463 | 0.75 | 0.83215 |
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Target: 5'- cGAGACgCGACGgauuggCUCAGAGGGAUGa -3' miRNA: 3'- -UUUUGaGUUGUaa----GAGUCUCCCUGCa -5' |
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| 2927 | 5' | -47.8 | NC_001493.1 | + | 58977 | 1.05 | 0.023023 |
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Target: 5'- gAAAACUCAACAUUCUCAGAGGGACGUc -3' miRNA: 3'- -UUUUGAGUUGUAAGAGUCUCCCUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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