Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29277 | 5' | -55.5 | NC_006150.1 | + | 123941 | 0.66 | 0.959171 |
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Target: 5'- aGGCUUCUGguacggGGaUUGccGCGGUGggUCUg -3' miRNA: 3'- aCCGAGGACa-----CC-GAC--UGCCACuaAGA- -5' |
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| 29277 | 5' | -55.5 | NC_006150.1 | + | 203007 | 0.66 | 0.955473 |
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Target: 5'- gUGGCUgCUGUugaGGCgggGAgGGUGGUg-- -3' miRNA: 3'- -ACCGAgGACA---CCGa--CUgCCACUAaga -5' |
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| 29277 | 5' | -55.5 | NC_006150.1 | + | 198001 | 0.66 | 0.947409 |
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Target: 5'- aGGCUCCUGgaGGCUcAUGGUGc---- -3' miRNA: 3'- aCCGAGGACa-CCGAcUGCCACuaaga -5' |
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| 29277 | 5' | -55.5 | NC_006150.1 | + | 120022 | 0.67 | 0.933602 |
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Target: 5'- gUGGCga-UGUGGCcuuccaUGACGGUGAa--- -3' miRNA: 3'- -ACCGaggACACCG------ACUGCCACUaaga -5' |
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| 29277 | 5' | -55.5 | NC_006150.1 | + | 111494 | 0.67 | 0.928536 |
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Target: 5'- -uGCUgCUGcUGGCUGGCGGcUGAgauggUCg -3' miRNA: 3'- acCGAgGAC-ACCGACUGCC-ACUa----AGa -5' |
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| 29277 | 5' | -55.5 | NC_006150.1 | + | 132417 | 0.69 | 0.872584 |
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Target: 5'- cGGCUCggaccuaUG-GGCUGGCGGUGGc--- -3' miRNA: 3'- aCCGAGg------ACaCCGACUGCCACUaaga -5' |
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| 29277 | 5' | -55.5 | NC_006150.1 | + | 23540 | 0.72 | 0.695474 |
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Target: 5'- cGGCcCCUGgacggGGUggaGACGGUGAUUUUa -3' miRNA: 3'- aCCGaGGACa----CCGa--CUGCCACUAAGA- -5' |
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| 29277 | 5' | -55.5 | NC_006150.1 | + | 91080 | 0.74 | 0.604822 |
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Target: 5'- aUGGCUCCUGcGGCacccGCGGUGAUgugugCUg -3' miRNA: 3'- -ACCGAGGACaCCGac--UGCCACUAa----GA- -5' |
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| 29277 | 5' | -55.5 | NC_006150.1 | + | 23358 | 1.08 | 0.00486 |
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Target: 5'- cUGGCUCCUGUGGCUGACGGUGAUUCUg -3' miRNA: 3'- -ACCGAGGACACCGACUGCCACUAAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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