Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29278 | 3' | -54.3 | NC_006150.1 | + | 102086 | 0.66 | 0.966149 |
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Target: 5'- --gCCUUUGUAGCACGUGaggaggacaCCUGAGa -3' miRNA: 3'- gaaGGAGACGUCGUGCAUg--------GGGUUCa -5' |
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| 29278 | 3' | -54.3 | NC_006150.1 | + | 117011 | 0.67 | 0.959366 |
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Target: 5'- uCUUCUugguUCUGCAGUuCGUGCCCgAGc- -3' miRNA: 3'- -GAAGG----AGACGUCGuGCAUGGGgUUca -5' |
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| 29278 | 3' | -54.3 | NC_006150.1 | + | 206429 | 0.67 | 0.955647 |
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Target: 5'- --cCCUCgucCAGC-CGUACCCCGAc- -3' miRNA: 3'- gaaGGAGac-GUCGuGCAUGGGGUUca -5' |
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| 29278 | 3' | -54.3 | NC_006150.1 | + | 80468 | 0.67 | 0.951702 |
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Target: 5'- aUUCCUCgGCgAGCGCGaGCuUCCAGGa -3' miRNA: 3'- gAAGGAGaCG-UCGUGCaUG-GGGUUCa -5' |
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| 29278 | 3' | -54.3 | NC_006150.1 | + | 110897 | 0.67 | 0.951702 |
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Target: 5'- -cUCCaUCcagGCGGUACGUauaAUCCCGAGUu -3' miRNA: 3'- gaAGG-AGa--CGUCGUGCA---UGGGGUUCA- -5' |
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| 29278 | 3' | -54.3 | NC_006150.1 | + | 184853 | 0.69 | 0.911747 |
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Target: 5'- -gUCCgagCUGCAGCGCaacGCCgCCAAGc -3' miRNA: 3'- gaAGGa--GACGUCGUGca-UGG-GGUUCa -5' |
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| 29278 | 3' | -54.3 | NC_006150.1 | + | 53885 | 0.7 | 0.86457 |
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Target: 5'- gCUUaCCUgUGCAaCACGUGCCCuCGGGa -3' miRNA: 3'- -GAA-GGAgACGUcGUGCAUGGG-GUUCa -5' |
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| 29278 | 3' | -54.3 | NC_006150.1 | + | 85013 | 0.74 | 0.682825 |
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Target: 5'- -cUCaUCUGCGGUACGUGCCCCc--- -3' miRNA: 3'- gaAGgAGACGUCGUGCAUGGGGuuca -5' |
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| 29278 | 3' | -54.3 | NC_006150.1 | + | 113830 | 0.74 | 0.662557 |
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Target: 5'- uUUCCgccgcagCgGCAGCugGUGCCUCAGGg -3' miRNA: 3'- gAAGGa------GaCGUCGugCAUGGGGUUCa -5' |
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| 29278 | 3' | -54.3 | NC_006150.1 | + | 31558 | 1.08 | 0.006412 |
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Target: 5'- gCUUCCUCUGCAGCACGUACCCCAAGUg -3' miRNA: 3'- -GAAGGAGACGUCGUGCAUGGGGUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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