Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29290 | 3' | -50.5 | NC_006150.1 | + | 191876 | 0.67 | 0.99668 |
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Target: 5'- uGCUGUGGUCUAcacugccuugaaCACAUGgGCGu -3' miRNA: 3'- gUGACACCAGGUuuag--------GUGUACgUGC- -5' |
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| 29290 | 3' | -50.5 | NC_006150.1 | + | 114116 | 0.67 | 0.995384 |
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Target: 5'- uCGCUG-GG-CUgaAAGUCCACGUGCAg- -3' miRNA: 3'- -GUGACaCCaGG--UUUAGGUGUACGUgc -5' |
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| 29290 | 3' | -50.5 | NC_006150.1 | + | 56238 | 0.68 | 0.993793 |
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Target: 5'- gACUGUGGcUCUAcuUUCAgCGUGCGCu -3' miRNA: 3'- gUGACACC-AGGUuuAGGU-GUACGUGc -5' |
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| 29290 | 3' | -50.5 | NC_006150.1 | + | 136165 | 0.71 | 0.948553 |
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Target: 5'- aGCUGUGGccgCCAGGUuuGCAuaaccgccucgaUGCACGa -3' miRNA: 3'- gUGACACCa--GGUUUAggUGU------------ACGUGC- -5' |
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| 29290 | 3' | -50.5 | NC_006150.1 | + | 125553 | 0.73 | 0.906112 |
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Target: 5'- cUACaG-GGUCCAAAaCCACAUGCAgGg -3' miRNA: 3'- -GUGaCaCCAGGUUUaGGUGUACGUgC- -5' |
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| 29290 | 3' | -50.5 | NC_006150.1 | + | 168388 | 0.83 | 0.445993 |
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Target: 5'- uGCUGguguUGGcCCGAGUCCACGUGCGCa -3' miRNA: 3'- gUGAC----ACCaGGUUUAGGUGUACGUGc -5' |
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| 29290 | 3' | -50.5 | NC_006150.1 | + | 55393 | 1.11 | 0.010531 |
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Target: 5'- aCACUGUGGUCCAAAUCCACAUGCACGg -3' miRNA: 3'- -GUGACACCAGGUUUAGGUGUACGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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