Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29306 | 3' | -52.6 | NC_006150.1 | + | 107087 | 0.7 | 0.945996 |
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Target: 5'- uUGggGAUGUC--CCGCGUCgUaCCCCg -3' miRNA: 3'- uGCuuUUACAGgaGGUGCAGgA-GGGG- -5' |
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| 29306 | 3' | -52.6 | NC_006150.1 | + | 183932 | 0.71 | 0.921401 |
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Target: 5'- -gGAGAAUGUCCUCCGCGagCggcaCCa -3' miRNA: 3'- ugCUUUUACAGGAGGUGCagGagg-GG- -5' |
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| 29306 | 3' | -52.6 | NC_006150.1 | + | 108314 | 0.72 | 0.897534 |
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Target: 5'- gACGAGGAggaagaagGUCCUCCAauuacUCCUCCUa -3' miRNA: 3'- -UGCUUUUa-------CAGGAGGUgc---AGGAGGGg -5' |
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| 29306 | 3' | -52.6 | NC_006150.1 | + | 34627 | 0.72 | 0.877255 |
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Target: 5'- uGCGuGAGU-UCUUCUggGUCCUCCCCu -3' miRNA: 3'- -UGCuUUUAcAGGAGGugCAGGAGGGG- -5' |
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| 29306 | 3' | -52.6 | NC_006150.1 | + | 121861 | 0.73 | 0.822719 |
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Target: 5'- cGCGGucccGUCCUCCGCaucuUCCUCCUCu -3' miRNA: 3'- -UGCUuuuaCAGGAGGUGc---AGGAGGGG- -5' |
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| 29306 | 3' | -52.6 | NC_006150.1 | + | 51987 | 0.74 | 0.805505 |
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Target: 5'- aGCGAAAccaggGUCCUCCuuugCCUCCCUg -3' miRNA: 3'- -UGCUUUua---CAGGAGGugcaGGAGGGG- -5' |
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| 29306 | 3' | -52.6 | NC_006150.1 | + | 180733 | 0.75 | 0.731183 |
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Target: 5'- -------cGUCCUCCuCGUCCUCCgCCa -3' miRNA: 3'- ugcuuuuaCAGGAGGuGCAGGAGG-GG- -5' |
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| 29306 | 3' | -52.6 | NC_006150.1 | + | 81411 | 1.13 | 0.005783 |
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Target: 5'- cACGAAAAUGUCCUCCACGUCCUCCCCg -3' miRNA: 3'- -UGCUUUUACAGGAGGUGCAGGAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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