Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29306 | 5' | -54.6 | NC_006150.1 | + | 138680 | 0.66 | 0.976611 |
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Target: 5'- uUGUUGGcucGGACGUUCAgcgucUGAGGAaggCCg -3' miRNA: 3'- gACGACU---CCUGCAAGU-----ACUCCUg--GGa -5' |
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| 29306 | 5' | -54.6 | NC_006150.1 | + | 88352 | 0.66 | 0.971404 |
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Target: 5'- -gGgaGGGGGCGgcgCAUGcAGGACgCCg -3' miRNA: 3'- gaCgaCUCCUGCaa-GUAC-UCCUG-GGa -5' |
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| 29306 | 5' | -54.6 | NC_006150.1 | + | 142169 | 0.67 | 0.954849 |
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Target: 5'- gUGCUGGGGAUGg--AUGAGGAUaCUUa -3' miRNA: 3'- gACGACUCCUGCaagUACUCCUG-GGA- -5' |
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| 29306 | 5' | -54.6 | NC_006150.1 | + | 81675 | 0.67 | 0.95089 |
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Target: 5'- --uUUGAGGGC---CGUGAGGACCCc -3' miRNA: 3'- gacGACUCCUGcaaGUACUCCUGGGa -5' |
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| 29306 | 5' | -54.6 | NC_006150.1 | + | 120215 | 0.68 | 0.932774 |
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Target: 5'- -aGCUacaccgugaucGGGGACGcgUCAUGAuGGCCCUa -3' miRNA: 3'- gaCGA-----------CUCCUGCa-AGUACUcCUGGGA- -5' |
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| 29306 | 5' | -54.6 | NC_006150.1 | + | 129607 | 0.68 | 0.922323 |
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Target: 5'- gUGCUGAaggcugugcguGGACGcUUCGUGGGGcgcgagguCCCUa -3' miRNA: 3'- gACGACU-----------CCUGC-AAGUACUCCu-------GGGA- -5' |
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| 29306 | 5' | -54.6 | NC_006150.1 | + | 145035 | 0.69 | 0.885416 |
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Target: 5'- -cGCUcaccGGGGA-GUUCGgucgGGGGACCCUg -3' miRNA: 3'- gaCGA----CUCCUgCAAGUa---CUCCUGGGA- -5' |
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| 29306 | 5' | -54.6 | NC_006150.1 | + | 216375 | 0.75 | 0.612957 |
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Target: 5'- -aGCUGAGGACGUUUAacAGGcCCCa -3' miRNA: 3'- gaCGACUCCUGCAAGUacUCCuGGGa -5' |
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| 29306 | 5' | -54.6 | NC_006150.1 | + | 81447 | 1.08 | 0.005998 |
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Target: 5'- cCUGCUGAGGACGUUCAUGAGGACCCUg -3' miRNA: 3'- -GACGACUCCUGCAAGUACUCCUGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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