Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29307 | 5' | -62.8 | NC_006150.1 | + | 139392 | 0.66 | 0.677978 |
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Target: 5'- -aCGGUCCGCcgC-CUGGGGuACGGGAg -3' miRNA: 3'- agGUCGGGCGa-GaGGCCCC-UGUCCUa -5' |
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| 29307 | 5' | -62.8 | NC_006150.1 | + | 75707 | 0.67 | 0.633812 |
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Target: 5'- cUCCAGCUccaccgugagcacauCGCUCUCCGGcGGAgacucgccccCAGGc- -3' miRNA: 3'- -AGGUCGG---------------GCGAGAGGCC-CCU----------GUCCua -5' |
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| 29307 | 5' | -62.8 | NC_006150.1 | + | 75024 | 0.67 | 0.629961 |
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Target: 5'- -gCGGCCCGCUCggaggaacugCCGGuGGugGGuGAc -3' miRNA: 3'- agGUCGGGCGAGa---------GGCC-CCugUC-CUa -5' |
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| 29307 | 5' | -62.8 | NC_006150.1 | + | 119466 | 0.68 | 0.562958 |
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Target: 5'- -aCAGCCUGCgcauuuaCUUCGGGGACGacccGGAa -3' miRNA: 3'- agGUCGGGCGa------GAGGCCCCUGU----CCUa -5' |
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| 29307 | 5' | -62.8 | NC_006150.1 | + | 430 | 0.69 | 0.525482 |
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Target: 5'- gUCGGCCCGU--UCCGGGaGGCuGGAg -3' miRNA: 3'- aGGUCGGGCGagAGGCCC-CUGuCCUa -5' |
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| 29307 | 5' | -62.8 | NC_006150.1 | + | 68685 | 0.69 | 0.480049 |
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Target: 5'- gCUAGCggugCUGCggugaCUUCGGGGACGGGAUa -3' miRNA: 3'- aGGUCG----GGCGa----GAGGCCCCUGUCCUA- -5' |
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| 29307 | 5' | -62.8 | NC_006150.1 | + | 24578 | 0.71 | 0.41989 |
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Target: 5'- uUCCAGCauagcucucUgGCUCUCCGGGGuguGGAUg -3' miRNA: 3'- -AGGUCG---------GgCGAGAGGCCCCuguCCUA- -5' |
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| 29307 | 5' | -62.8 | NC_006150.1 | + | 113381 | 0.71 | 0.395539 |
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Target: 5'- gCCGGgCUGCUUUCUGGGGAgaaagUAGGGUa -3' miRNA: 3'- aGGUCgGGCGAGAGGCCCCU-----GUCCUA- -5' |
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| 29307 | 5' | -62.8 | NC_006150.1 | + | 88384 | 0.73 | 0.288205 |
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Target: 5'- aUCCAGCCCGCgg--CGGGGugGGGu- -3' miRNA: 3'- -AGGUCGGGCGagagGCCCCugUCCua -5' |
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| 29307 | 5' | -62.8 | NC_006150.1 | + | 81965 | 1.05 | 0.001958 |
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Target: 5'- cUCCAGCCCGCUCUCCGGGGACAGGAUc -3' miRNA: 3'- -AGGUCGGGCGAGAGGCCCCUGUCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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