Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29309 | 3' | -54.5 | NC_006150.1 | + | 55373 | 0.66 | 0.982699 |
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Target: 5'- aCUUaAUGAGCUCGCuGAccacacugUGGUCCa-- -3' miRNA: 3'- gGAAcUACUCGAGCG-CU--------ACCAGGcgc -5' |
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| 29309 | 3' | -54.5 | NC_006150.1 | + | 135522 | 0.67 | 0.971042 |
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Target: 5'- aCCUUG--GAGgUCGCcAUGGUgccgCCGCGg -3' miRNA: 3'- -GGAACuaCUCgAGCGcUACCA----GGCGC- -5' |
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| 29309 | 3' | -54.5 | NC_006150.1 | + | 102897 | 0.68 | 0.954599 |
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Target: 5'- gUCUUGAUaGGCauaguaauUCGCGGUGG-CCGUa -3' miRNA: 3'- -GGAACUAcUCG--------AGCGCUACCaGGCGc -5' |
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| 29309 | 3' | -54.5 | NC_006150.1 | + | 86270 | 0.68 | 0.943945 |
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Target: 5'- -aUUGAUaGGCgccuucgaaaauagCGUGAUGGUUCGCGg -3' miRNA: 3'- ggAACUAcUCGa-------------GCGCUACCAGGCGC- -5' |
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| 29309 | 3' | -54.5 | NC_006150.1 | + | 132425 | 0.74 | 0.714019 |
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Target: 5'- aCCU--AUGGGCUgGCGGUGGccgaggaguuugucUCCGCGg -3' miRNA: 3'- -GGAacUACUCGAgCGCUACC--------------AGGCGC- -5' |
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| 29309 | 3' | -54.5 | NC_006150.1 | + | 85064 | 1.12 | 0.004559 |
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Target: 5'- gCCUUGAUGAGCUCGCGAUGGUCCGCGg -3' miRNA: 3'- -GGAACUACUCGAGCGCUACCAGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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