Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29316 | 3' | -48 | NC_006150.1 | + | 92675 | 0.66 | 0.999944 |
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Target: 5'- uGACGUGU----GCCUUCGGgGUGCg -3' miRNA: 3'- uUUGCACAuuggCGGAAGUUgCAUGa -5' |
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| 29316 | 3' | -48 | NC_006150.1 | + | 27483 | 0.66 | 0.999903 |
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Target: 5'- -----aGUGGCCGCCggauagcgUgAGCGUGCUg -3' miRNA: 3'- uuugcaCAUUGGCGGa-------AgUUGCAUGA- -5' |
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| 29316 | 3' | -48 | NC_006150.1 | + | 120617 | 0.68 | 0.999343 |
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Target: 5'- cGAGCG-GUGACCGCUUaaAACGcUACg -3' miRNA: 3'- -UUUGCaCAUUGGCGGAagUUGC-AUGa -5' |
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| 29316 | 3' | -48 | NC_006150.1 | + | 150119 | 0.68 | 0.999343 |
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Target: 5'- uAGACGacugGU-GCCGCCga-GGCGUGCUg -3' miRNA: 3'- -UUUGCa---CAuUGGCGGaagUUGCAUGA- -5' |
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| 29316 | 3' | -48 | NC_006150.1 | + | 92475 | 0.69 | 0.997888 |
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Target: 5'- gGGGCGUGUGcUCGCUUUCGugGUccagGCa -3' miRNA: 3'- -UUUGCACAUuGGCGGAAGUugCA----UGa -5' |
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| 29316 | 3' | -48 | NC_006150.1 | + | 152627 | 0.69 | 0.997014 |
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Target: 5'- -----cGUGGCCGCCUUCAA-GUAUUg -3' miRNA: 3'- uuugcaCAUUGGCGGAAGUUgCAUGA- -5' |
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| 29316 | 3' | -48 | NC_006150.1 | + | 180378 | 0.71 | 0.990069 |
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Target: 5'- -cACGUGuUGGCCGCCaaCAgcACGUGCg -3' miRNA: 3'- uuUGCAC-AUUGGCGGaaGU--UGCAUGa -5' |
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| 29316 | 3' | -48 | NC_006150.1 | + | 94716 | 0.72 | 0.981514 |
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Target: 5'- gAAAgGUGUuaauCUGCCUgUCAACGUGCa -3' miRNA: 3'- -UUUgCACAuu--GGCGGA-AGUUGCAUGa -5' |
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| 29316 | 3' | -48 | NC_006150.1 | + | 108753 | 1.07 | 0.031271 |
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Target: 5'- cAAACGUGUAACCGCCUUCAACGUACUg -3' miRNA: 3'- -UUUGCACAUUGGCGGAAGUUGCAUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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