Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29317 | 3' | -50.1 | NC_006150.1 | + | 154287 | 0.66 | 0.999372 |
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Target: 5'- uGUCguUGcugGGGCUGUUGUUGuGUuGCu -3' miRNA: 3'- cUAGguACa--CCUGACAACAGCuCA-CG- -5' |
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| 29317 | 3' | -50.1 | NC_006150.1 | + | 219074 | 0.67 | 0.998339 |
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Target: 5'- gGAUCCcaAUGgGGAUUGUaGUCGccggccgcccaGGUGCg -3' miRNA: 3'- -CUAGG--UACaCCUGACAaCAGC-----------UCACG- -5' |
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| 29317 | 3' | -50.1 | NC_006150.1 | + | 103854 | 0.67 | 0.997638 |
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Target: 5'- -cUCCAUGcUGGcgucuaaaAUUGUUGgcaugaCGAGUGCg -3' miRNA: 3'- cuAGGUAC-ACC--------UGACAACa-----GCUCACG- -5' |
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| 29317 | 3' | -50.1 | NC_006150.1 | + | 109181 | 0.67 | 0.997638 |
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Target: 5'- cGUCCAgc-GGGCUG-UGUUGcGUGCa -3' miRNA: 3'- cUAGGUacaCCUGACaACAGCuCACG- -5' |
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| 29317 | 3' | -50.1 | NC_006150.1 | + | 61106 | 0.67 | 0.997638 |
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Target: 5'- cGAUCCuaugaGUGUGGAUcuuUGUUGuUCGAGa-- -3' miRNA: 3'- -CUAGG-----UACACCUG---ACAAC-AGCUCacg -5' |
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| 29317 | 3' | -50.1 | NC_006150.1 | + | 201688 | 0.68 | 0.992996 |
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Target: 5'- --aCC-UGUGGAauaauuCUGUUGgaCGAGUGCg -3' miRNA: 3'- cuaGGuACACCU------GACAACa-GCUCACG- -5' |
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| 29317 | 3' | -50.1 | NC_006150.1 | + | 169134 | 0.68 | 0.992996 |
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Target: 5'- uGAUCUAUG-GuGugUGUUG-CGuGUGCa -3' miRNA: 3'- -CUAGGUACaC-CugACAACaGCuCACG- -5' |
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| 29317 | 3' | -50.1 | NC_006150.1 | + | 111053 | 1.13 | 0.009228 |
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Target: 5'- aGAUCCAUGUGGACUGUUGUCGAGUGCu -3' miRNA: 3'- -CUAGGUACACCUGACAACAGCUCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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