Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29318 | 3' | -55.7 | NC_006150.1 | + | 181394 | 0.66 | 0.96383 |
Target: 5'- aACAGGGUGauggcaugCGCUUCuggGUGAaaGCGAgCGu -3' miRNA: 3'- -UGUCCCGCa-------GCGAAG---CACU--UGCUgGC- -5' |
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29318 | 3' | -55.7 | NC_006150.1 | + | 124090 | 0.66 | 0.96383 |
Target: 5'- aGCAGguGGCGUCGCUagUUGUGuugguCGACg- -3' miRNA: 3'- -UGUC--CCGCAGCGA--AGCACuu---GCUGgc -5' |
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29318 | 3' | -55.7 | NC_006150.1 | + | 219801 | 0.66 | 0.953185 |
Target: 5'- gGCGGGcGCGUUGUggCGUGAAUGuuUGu -3' miRNA: 3'- -UGUCC-CGCAGCGaaGCACUUGCugGC- -5' |
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29318 | 3' | -55.7 | NC_006150.1 | + | 12819 | 0.67 | 0.940611 |
Target: 5'- ---cGGUGUaUGCagCGUGGACGGCCGg -3' miRNA: 3'- ugucCCGCA-GCGaaGCACUUGCUGGC- -5' |
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29318 | 3' | -55.7 | NC_006150.1 | + | 217601 | 0.68 | 0.92604 |
Target: 5'- cGCAGGGCGgugCGCU-CuUG-GCGACUGc -3' miRNA: 3'- -UGUCCCGCa--GCGAaGcACuUGCUGGC- -5' |
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29318 | 3' | -55.7 | NC_006150.1 | + | 128298 | 0.73 | 0.686437 |
Target: 5'- uCAGGGCGUCgaGCUUC-UGAuACGACUGc -3' miRNA: 3'- uGUCCCGCAG--CGAAGcACU-UGCUGGC- -5' |
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29318 | 3' | -55.7 | NC_006150.1 | + | 117963 | 1.09 | 0.004854 |
Target: 5'- gACAGGGCGUCGCUUCGUGAACGACCGg -3' miRNA: 3'- -UGUCCCGCAGCGAAGCACUUGCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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