miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
29318 3' -55.7 NC_006150.1 + 181394 0.66 0.96383
Target:  5'- aACAGGGUGauggcaugCGCUUCuggGUGAaaGCGAgCGu -3'
miRNA:   3'- -UGUCCCGCa-------GCGAAG---CACU--UGCUgGC- -5'
29318 3' -55.7 NC_006150.1 + 124090 0.66 0.96383
Target:  5'- aGCAGguGGCGUCGCUagUUGUGuugguCGACg- -3'
miRNA:   3'- -UGUC--CCGCAGCGA--AGCACuu---GCUGgc -5'
29318 3' -55.7 NC_006150.1 + 219801 0.66 0.953185
Target:  5'- gGCGGGcGCGUUGUggCGUGAAUGuuUGu -3'
miRNA:   3'- -UGUCC-CGCAGCGaaGCACUUGCugGC- -5'
29318 3' -55.7 NC_006150.1 + 12819 0.67 0.940611
Target:  5'- ---cGGUGUaUGCagCGUGGACGGCCGg -3'
miRNA:   3'- ugucCCGCA-GCGaaGCACUUGCUGGC- -5'
29318 3' -55.7 NC_006150.1 + 217601 0.68 0.92604
Target:  5'- cGCAGGGCGgugCGCU-CuUG-GCGACUGc -3'
miRNA:   3'- -UGUCCCGCa--GCGAaGcACuUGCUGGC- -5'
29318 3' -55.7 NC_006150.1 + 128298 0.73 0.686437
Target:  5'- uCAGGGCGUCgaGCUUC-UGAuACGACUGc -3'
miRNA:   3'- uGUCCCGCAG--CGAAGcACU-UGCUGGC- -5'
29318 3' -55.7 NC_006150.1 + 117963 1.09 0.004854
Target:  5'- gACAGGGCGUCGCUUCGUGAACGACCGg -3'
miRNA:   3'- -UGUCCCGCAGCGAAGCACUUGCUGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.