Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2932 | 5' | -57.4 | NC_001493.1 | + | 52417 | 0.67 | 0.790569 |
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Target: 5'- gGGAaGGUCu--UCCCugucaacuGCCUGGGUCu -3' miRNA: 3'- gCCUaUCAGuguAGGGc-------CGGACCCAG- -5' |
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| 2932 | 5' | -57.4 | NC_001493.1 | + | 90504 | 0.67 | 0.762699 |
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Target: 5'- gGGGUGGUCAUcgcacaCCgCGGUC-GGGUCg -3' miRNA: 3'- gCCUAUCAGUGua----GG-GCCGGaCCCAG- -5' |
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| 2932 | 5' | -57.4 | NC_001493.1 | + | 75334 | 0.68 | 0.737697 |
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Target: 5'- -uGAUGGUaCACAUCUucgccaucgugcucuUGGCCUGGGcCa -3' miRNA: 3'- gcCUAUCA-GUGUAGG---------------GCCGGACCCaG- -5' |
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| 2932 | 5' | -57.4 | NC_001493.1 | + | 126797 | 0.68 | 0.723968 |
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Target: 5'- aCGGGUcGUCGagccCUCGGUCUGGGUg -3' miRNA: 3'- -GCCUAuCAGUgua-GGGCCGGACCCAg -5' |
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| 2932 | 5' | -57.4 | NC_001493.1 | + | 11243 | 0.68 | 0.723968 |
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Target: 5'- aCGGGUcGUCGagccCUCGGUCUGGGUg -3' miRNA: 3'- -GCCUAuCAGUgua-GGGCCGGACCCAg -5' |
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| 2932 | 5' | -57.4 | NC_001493.1 | + | 80298 | 0.68 | 0.694061 |
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Target: 5'- gCGGggGGaUCcCGUCCaUGGCCaGGGUCg -3' miRNA: 3'- -GCCuaUC-AGuGUAGG-GCCGGaCCCAG- -5' |
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| 2932 | 5' | -57.4 | NC_001493.1 | + | 61429 | 1.08 | 0.002109 |
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Target: 5'- uCGGAUAGUCACAUCCCGGCCUGGGUCc -3' miRNA: 3'- -GCCUAUCAGUGUAGGGCCGGACCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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