Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29321 | 3' | -51.2 | NC_006150.1 | + | 114719 | 0.7 | 0.971322 |
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Target: 5'- cUCCUcCGAGGGCGGccGAGCcaugUCUCGCc -3' miRNA: 3'- -AGGAaGCUUCCGCU--UUUGu---GGAGCGc -5' |
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| 29321 | 3' | -51.2 | NC_006150.1 | + | 126447 | 0.7 | 0.968379 |
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Target: 5'- -gCUUUGAuuucGCGAu-ACACCUCGCGa -3' miRNA: 3'- agGAAGCUuc--CGCUuuUGUGGAGCGC- -5' |
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| 29321 | 3' | -51.2 | NC_006150.1 | + | 120023 | 0.7 | 0.968379 |
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Target: 5'- aCCUgggCG-AGGCcguAACACCUCGCc -3' miRNA: 3'- aGGAa--GCuUCCGcuuUUGUGGAGCGc -5' |
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| 29321 | 3' | -51.2 | NC_006150.1 | + | 204974 | 0.71 | 0.950431 |
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Target: 5'- cCCUUCGAGcGCGAAgagcgucgucAGCGCUUCGUa -3' miRNA: 3'- aGGAAGCUUcCGCUU----------UUGUGGAGCGc -5' |
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| 29321 | 3' | -51.2 | NC_006150.1 | + | 202816 | 0.72 | 0.936915 |
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Target: 5'- gUCCUgcauUCGAgggauggguggAGGCGGAGGCGCUugaagCGCGg -3' miRNA: 3'- -AGGA----AGCU-----------UCCGCUUUUGUGGa----GCGC- -5' |
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| 29321 | 3' | -51.2 | NC_006150.1 | + | 47705 | 0.72 | 0.926713 |
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Target: 5'- gUCCUccUCGAGGacCGGcucACACCUCGCGg -3' miRNA: 3'- -AGGA--AGCUUCc-GCUuu-UGUGGAGCGC- -5' |
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| 29321 | 3' | -51.2 | NC_006150.1 | + | 167857 | 0.73 | 0.909611 |
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Target: 5'- gUCCUUCGGAGGCac-AGCGCCauuacCGCa -3' miRNA: 3'- -AGGAAGCUUCCGcuuUUGUGGa----GCGc -5' |
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| 29321 | 3' | -51.2 | NC_006150.1 | + | 109813 | 0.74 | 0.869096 |
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Target: 5'- cUCCUggGAAGGCGAcGACgACCUC-CGg -3' miRNA: 3'- -AGGAagCUUCCGCUuUUG-UGGAGcGC- -5' |
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| 29321 | 3' | -51.2 | NC_006150.1 | + | 134193 | 1.09 | 0.012978 |
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Target: 5'- cUCCUUCGAAGGCGAAAACACCUCGCGc -3' miRNA: 3'- -AGGAAGCUUCCGCUUUUGUGGAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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