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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29325 | 5' | -57.7 | NC_006150.1 | + | 816 | 0.66 | 0.887972 |
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Target: 5'- --cGCUGagucaGCCGGGCGGCUCgGGu -3' miRNA: 3'- uauUGACgaa--CGGCUCGCCGGGaCCu -5' |
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| 29325 | 5' | -57.7 | NC_006150.1 | + | 157385 | 0.67 | 0.835953 |
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Target: 5'- -gGGCUGCUauugcUGCUGAggGUGGUgCUGGGa -3' miRNA: 3'- uaUUGACGA-----ACGGCU--CGCCGgGACCU- -5' |
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| 29325 | 5' | -57.7 | NC_006150.1 | + | 88492 | 0.68 | 0.793318 |
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Target: 5'- -cAGCcgGCUUGCCccGaCGaGCCCUGGAc -3' miRNA: 3'- uaUUGa-CGAACGGcuC-GC-CGGGACCU- -5' |
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| 29325 | 5' | -57.7 | NC_006150.1 | + | 428 | 0.68 | 0.784337 |
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Target: 5'- ---uCUGUUUGCCGcggugucacuGCGGCCCUGc- -3' miRNA: 3'- uauuGACGAACGGCu---------CGCCGGGACcu -5' |
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| 29325 | 5' | -57.7 | NC_006150.1 | + | 170300 | 0.7 | 0.658716 |
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Target: 5'- uAUGACgagaucgGuuGAGCGGUCCUGGAa -3' miRNA: 3'- -UAUUGacgaa--CggCUCGCCGGGACCU- -5' |
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| 29325 | 5' | -57.7 | NC_006150.1 | + | 147634 | 1.06 | 0.003631 |
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Target: 5'- uAUAACUGCUUGCCGAGCGGCCCUGGAu -3' miRNA: 3'- -UAUUGACGAACGGCUCGCCGGGACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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